食品科学 ›› 2020, Vol. 41 ›› Issue (14): 146-152.doi: 10.7506/spkx1002-6630-20190514-147

• 生物工程 • 上一篇    下一篇

1 株副溶血性弧菌裂解性噬菌体VpJYP2的生物学特性及应用

江艳华,王联珠,李风铃,曲 梦,郭莹莹,翟毓秀,姚 琳   

  1. (中国水产科学研究院黄海水产研究所,农业农村部水产品质量安全检测与评价重点实验室,山东 青岛 266071)
  • 发布日期:2020-07-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFC1600703);国家自然科学基金青年科学基金项目(31601566); 国家农产品质量安全风险评估重大专项(GJFP2018009)

Biological Properties and Application of a Lytic Vibrio parahaemolyticus Phage

JIANG Yanhua, WANG Lianzhu, LI Fengling, QU Meng, GUO Yingying, ZHAI Yuxiu, YAO Lin   

  1. (Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China)
  • Published:2020-07-29

摘要: 对1 株副溶血性弧菌裂解性噬菌体VpJYP2进行生物学特性分析,探讨VpJYP2对生三文鱼片中副溶血性弧菌的杀菌效果。结果显示,噬菌体VpJYP2的噬菌斑透明,直径为1~2 mm,外有晕圈;透射电镜观察显示,其头部呈二十面体立体结构,直径约64 nm,尾部长约70 nm,尾鞘带宽约20 nm,属于肌尾病毒科(Myoviridae);VpJYP2基因组全长25 363 bp,含有45 个开放阅读框,推定为已知功能的有13 个;VpJYP2在40~50 ℃稳定,在pH 4~11稳定,最佳感染复数为0.01,感染宿主菌的潜伏期约为5 min,裂解期约为55 min,裂解量约为45。VpJYP2能显著降低生三文鱼片中副溶血性弧菌的含量。结果表明,VpJYP2具有作为副溶血性弧菌生物杀菌剂的应用潜力。

关键词: 副溶血性弧菌;裂解性噬菌体;生物学特性;杀菌作用;生三文鱼

Abstract: The biological properties of a lytic phage VpJYP2 that infects Vibrio parahaemolyticus were determined, and its bactericidal effect against V. parahaemolyticus in raw salmon fillets was analyzed as well. The results showed that the plaques of VpJYP2 were clear and about 1?2 mm in diameter with halos around them. VpJYP2 had an icosahedral head of about 64 nm in diameter and a tail of about 70 nm in length, with a tail sheath of 20 nm in width. VpJYP2 was identified to belong to the family Myoviridae. VpJYP2 had a genome of 25 363 bp containing 45 open reading frames (ORF), 13 of which were predicted to encode certain functional proteins. VpJYP2 was stable at 40?50 ℃ and under pH 4?11. Its optimal multiplicity of infection was 0.01. One-step growth experiments showed that its latent time and burst time were 5 and 55 min, respectively, and burst size was 45. VpJYP2 significantly reduced V. parahaemolyticus in raw salmon fillets. These results indicate that VpJYP2 can serve as a promising biocontrol agent against V. parahaemolyticus.

Key words: Vibrio parahaemolyticus; lytic phage; biological properties; bactericidal effect; raw salmon

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