食品科学 ›› 2020, Vol. 41 ›› Issue (20): 61-67.doi: 10.7506/spkx1002-6630-20190515-173

• 食品化学 • 上一篇    下一篇

小球藻多糖的分离纯化及理化性质

史瑞琴,梁静静,李大伟,王颉,郭书贤,马艳莉   

  1. (1.南阳理工学院 河南省工业微生物资源与发酵技术重点实验室,河南 南阳 473004;2.河北农业大学食品科技学院,河北 保定 071000)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    国家海洋公益性行业科研专项(201205031);河北省科技计划项目(15273204D); 河南省工业微生物资源与发酵技术重点实验室开放课题(IMRFT20180308)

Isolation, Purification and Physicochemical Properties of Polysaccharides from Chlorella

SHI Ruiqin, LIANG Jingjing, LI Dawei, WANG Jie, GUO Shuxian, MA Yanli   

  1. (1. Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang Institute of Technology, Nanyang 473004, China; 2. College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 通过对比2 种不同脱蛋白方法,确定较适宜的脱蛋白方法并以DEAE-Sepharose Fast Flow柱层析获得的多糖F1、F2、F3为主要原料,研究多糖的微观结构、单糖组成、刚果红反应及流变学性质。结果表明:不同多糖组分呈现不同的形态特征,主要以片状与纤维细丝状为主;F1与F2均以半乳糖为主要单糖,而F3中阿拉伯糖的含量最高;3 种组分均以无规则线团链构象存在于水溶液中。静态与动态流变学性质研究表明:小球藻多糖为假塑性流体,且为非凝胶性多糖。本实验可为藻类产品的研发与应用提供参考。

关键词: 小球藻;多糖;理化性质;流变学

Abstract: In this study, the crude polysaccharides extracted from Chlorella were deproteinized by Sevag method and then fractionated by DEAE-Sepharose Fast flow column chromatography into three fractions (F1, F2, and F3). The microstructure, monosaccharide composition, congo red reactivity, and rheological properties of the purified polysaccharides were investigated. The results showed that these three polysaccharides exhibited different morphological characteristics, mainly existing in the form of flakes or filaments. The monosaccharide composition of F1 and F2 consisted mainly of galactose (Gal), while arabinose (Ara) was the most abundant monosaccharide in F3. All three polysaccharides were present in aqueous solutions in a random coil conformation. Static and dynamic rheological properties showed that these polysaccharides were pseudoplastic fluids and non-gelling polysaccharides. This study will provide reference for the development and application of algae products.

Key words: Chlorella; polysaccharides; physicochemical properties; rheological properties

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