食品科学 ›› 2020, Vol. 41 ›› Issue (22): 177-183.doi: 10.7506/spkx1002-6630-20190929-360

• 生物工程 • 上一篇    下一篇

发酵型青腐乳菌群结构与风味物质及其相关性分析

孙娜,张雅婷,于寒松,朱先明,朱世杰,周树辉,任大勇   

  1. (1.吉林农业大学食品科学与工程学院,吉林 长春 130118;2.长春市朱老六食品股份有限公司,吉林 长春 130500)
  • 出版日期:2020-11-25 发布日期:2020-11-26
  • 基金资助:
    吉林省科技发展计划重点研发项目(20200402068NC);朱老六产品质量与安全研发计划项目(横20190072); 吉林农业大学大学生科技创新基金项目(2018088)

Microflora Structure and Flavor Components and Correlation between Them in Fermented Stinky Tofu

SUN Na, ZHANG Yating, YU Hansong, ZHU Xianming, ZHU Shijie, ZHOU Shuhui, REN Dayong   

  1. (1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;2. Changchun Zhu Laoliu Food Co. Ltd., Changchun 130500, China)
  • Online:2020-11-25 Published:2020-11-26

摘要: 通过高通量测序技术及气相色谱-质谱联用方法,对发酵前期青腐乳和发酵末期青腐乳卤水中的微生物多样性及风味成分进行分析,在此基础上探讨菌群结构与风味物质之间的相关性。结果表明,微生物在属水平上差异显著,尤其是明串珠菌属(Leuconostoc)和盐厌氧菌属(Halanaerobium)。发酵末期青腐乳与发酵前期青腐乳卤水中分别含27 种和45 种挥发性成分,两者有6 种相同的成分,只有发酵末期青腐乳中检测出了提供臭味的含硫化合物及吲哚。菌属与风味物质相关性分析表明,Leuconostoc、Halanaerobium与含硫化合物、吲哚紧密相关,是臭味的主要来源;乳杆菌属(Lactobacillus)、四联球菌属(Tetragenococcus)和Chishuiella与酯类、酮类、醇类相关性最大,为青腐乳提供香气。

关键词: 青腐乳;风味成分;菌群结构;相关性

Abstract: High-throughput sequencing technology and gas chromatography and mass spectrometry (GC-MS) were respectively used to analyze the microbial diversity and flavor components of fermented?stinky?tofu?brine at the beginning and end of fermentation. Further, the correlation between them was analyzed. The results indicated that the microbial community structure changed significantly at the genus level, especially for the genera Leuconostoc and Halanaerobium. In total, 45 and 27 volatile components were found to exist at the beginning and end of fermentation, respectively. Six volatile component were common to both. The offensive odorants sulfur-containing compounds and indole were detected exclusively at the end of fermentation. The correlation analysis indicated that Leuconostoc and Halanoerobium as the main sources of stinky odor were closely related to sulfur-containing compounds and indole. Lactobacillus, Tetragenococcus and Chishuiella were most correlated with esters, ketones, and alcohols, imparting the aroma to fermented stinky tofu.

Key words: fermented stinky tofu; flavor components; microflora structure; correlation

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