食品科学 ›› 2020, Vol. 41 ›› Issue (24): 209-215.doi: 10.7506/spkx1002-6630-20190929-351

• 成分分析 • 上一篇    下一篇

华东覆盆子果、茎与叶的酚类成分及抗氧化活性分析

陈青青,李柯,唐晓清,耿丽,王磊,彭雅萍   

  1. (1.南京农业大学园艺学院,江苏 南京 210095;2.句容市茅山仙草中药材专业合作社,江苏 句容 212404)
  • 出版日期:2020-12-25 发布日期:2020-12-28
  • 基金资助:
    中医药公共卫生服务补助专项(财社〔2018〕43号);中央高校基本科研业务费专项(KYZZ201913); 2019年医疗服务与保障能力提升补助资金项目(财社〔2019〕39号)

Analysis of Phenolic Composition and Antioxidant Activities in Fruits, Stems and Leaves of Rubus chingii Hu

CHEN Qingqing, LI Ke, TANG Xiaoqing, GENG Li, WANG Lei, PENG Yaping   

  1. (1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2. Chinese Herbal Medicine Cooperative of Jurong Maoshan Xiancao, Jurong 212404, China)
  • Online:2020-12-25 Published:2020-12-28

摘要: 为探究华东覆盆子不同部位酚类成分及抗氧化活性,本实验采用分光光度法及超高效液相色谱-质谱联用技术对其果、茎及叶(嫩叶与老叶)的醇提取物中酚类化合物进行分析,并测定其抗氧化活性。结果表明:嫩叶的总酚含量最高(69.49 mg/g),老叶的总黄酮含量最高(87.21 mg/g),果的原花青素含量最高(36.70 mg/g)。嫩叶与老叶的酚类化合物组成相似,主要为山柰酚衍生物和槲皮素及其衍生物,与果和茎中的黄酮类化合物差异明显。嫩叶的抗氧化活性最强,老叶和茎次之,果的抗氧化活性最弱。相关性分析显示,总酚对抗氧化活性贡献程度最高。可见华东覆盆子嫩叶的酚类化合物可以作为潜在天然抗氧化剂来源,具有一定的保健品开发潜力。本实验为茎叶的开发利用提供理论参考,从而促进华东覆盆子的综合利用。

关键词: 华东覆盆子;酚类化合物;抗氧化活性

Abstract: The phenolic compounds in the fruits, stems and leaves (tender leaves and old leaves) of Rubus chingii Hu were separated and detected by ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Their antioxidant activities were determined by spectrophotometry. The results showed that the contents of total phenol, total flavonoid and proanthocyanidins in the above-ground parts were significantly different, the highest contents of total phenol (69.49 mg/g), total flavonoid (87.21 mg/g) and proanthocyanidins (36.70 mg/g) being in young leaves, old leaves and fruits, respectively. The composition of phenolic compounds in young leaves was similar to that in old leaves, mainly including kaempferol derivatives as well as quercetin and its derivatives, but significantly differed from that in fruits and stems. The antioxidant activities of the three parts of R. chingii Hu followed the decreasing order of young leaves > old leaves and stems > fruits. According to correlation analysis, the total phenolics contributed to the antioxidant activity. From this study, it could be seen that the young leaves of R. chingii Hu are a potential source of natural phenolic antioxidants that is promising for the development of health foods. This study provides theoretical reference for the development and utilization of this plant’s stems and leaves to promote its comprehensive utilization.

Key words: Rubus chingii Hu; phenolic compounds; antioxidant activity

中图分类号: