食品科学 ›› 2020, Vol. 41 ›› Issue (4): 214-221.doi: 10.7506/spkx1002-6630-20190110-136

• 成分分析 • 上一篇    下一篇

SDE和HS-SPME结合GC-MS分析霍山黄大茶香气成分的比较

朱晓凤,刘政权,宛晓春,陶勐,肖志鹏,程淑华   

  1. (安徽农业大学茶树生物学与资源利用国家重点实验室,安徽 合肥 230036)
  • 出版日期:2020-02-25 发布日期:2020-03-02
  • 基金资助:
    安徽省科技重大专项(18030701141);现代农业(茶叶)产业技术体系建设专项(CARS-23); 2016—2020年安徽省茶产业体系茶叶深加工岗位专家项目(11250009)

Comparison of Simultaneous Distillation and Extraction and Headspace-Solid Phase Microextraction for Analysis of Aroma Components of Huoshan Large-Leaf Yellow Tea by Gas Chromatography-Mass Spectrometry

ZHU Xiaofeng, LIU Zhengquan, WAN Xiaochun, TAO Meng, XIAO Zhipeng, CHENG Shuhua   

  1. (State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China)
  • Online:2020-02-25 Published:2020-03-02

摘要: 分别采用同时蒸馏萃取法(simultaneous distillation and extraction,SDE)和顶空固相微萃取法(headspace-solid phase microextraction,HS-SPME)提取霍山黄大茶中的香气成分,通过气相色谱-质谱定性定量分析,对2 种方法所得的香气成分进行比较。结果表明,2 种方法提取的香气成分和相对含量有很大差异,SDE法检测出的挥发性成分有74 种,HS-SPME法检测到的挥发性成分有76 种,2 种方法共同检出的组分有44 种。糠醛、5-甲基呋喃醛、3-乙基-2,5-二甲基吡嗪、水杨酸甲酯等是构成霍山黄大茶香气的重要物质,2 种方法测出的相对含量都在2%以上。2 种方法特有香气化合物分别有30、32 种,苯乙烯、苯乙醛、顺式芳樟醇氧化物(呋喃)、亚麻酸等仅在SDE法中检出,HS-SPME法中特有苯甲醛、十四烷、香叶基丙酮等香气组分。

关键词: 霍山黄大茶, 香气, 同时蒸馏萃取法, 顶空固相微萃取, 气相色谱-质谱

Abstract: Comparative analysis of the aroma components of Huoshan large-leaf yellow tea was performed by simultaneous distillation and extraction (SDE) and headspace-solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that the composition and contents of volatile compounds detected by the two methods were significantly different. A total of 74 compounds were obtained by SDE while 76 compounds by HS-SPME. In total, 44 components were detected by both methods. Furfural, 5-methyIfurfural, 3-ethyl-2,5-dimethylpyrazine and methyl salicylate were important substances that contributed to the unique aroma of large-leaf yellow tea and their relative contents were all over 2% obtained by both methods. Furthermore, 30 (styrene, benzeneacetaldehyde, cis-linaloloxide (furanoid), and linolenicacid) and 32 (benzaldehyde, tetradecane and geranylacetone) aroma components were exclusively detected by SDE and HS-SPME, respectively.

Key words: Huoshan large-leaf yellow tea, aroma, simultaneous distillation and extraction, headspace-solid phase microextraction, gas chromatography-mass spectrometry

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