食品科学 ›› 2021, Vol. 42 ›› Issue (13): 114-120.doi: 10.7506/spkx1002-6630-20201009-051

• 营养卫生 • 上一篇    下一篇

(+)-儿茶素与表没食子儿茶素没食子酸酯对乙醇诱导HepG2细胞脂代谢紊乱及氧化应激的影响

胡博然,丁建才,曹杨,田颖,国凤华,袁静   

  1. (1.扬州大学食品科学与工程学院,江苏 扬州 225127;2.首都儿科研究所,北京 100020;3.通化葡萄酒股份有限公司,吉林 通化 134002)
  • 出版日期:2021-07-15 发布日期:2021-07-27
  • 基金资助:
    国家自然科学基金面上项目(31370093)

Effects of (+)-Catechin and Epigallocatechin Gallate on Ethanol-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

HU Boran, DING Jiancai, CAO Yang, TIAN Ying, GUO Fenghua, YUAN Jing   

  1. (1. School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; 2. Capital Institute of Pediatrics, Beijing 100020, China; 3. Tonghua Winery Co., Ltd., Tonghua 134002, China)
  • Online:2021-07-15 Published:2021-07-27

摘要: 目的:体外对比(+)-儿茶素((+)-catechin,(+)-Cat)与表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对乙醇诱导的HepG2细胞脂代谢紊乱及氧化应激的差异。方法:以HepG2细胞为实验对象,乙醇作用组(ETOH组)加入作用浓度300 mmol/L乙醇,(+)-Cat+ETOH组加入200 μmol/L (+)-Cat和300 mmol/L乙醇,EGCG+ETOH组加入200 μmol/L EGCG和300 mmol/L乙醇;另设相同浓度的(+)-Cat组、EGCG组及正常组,各组均在37 ℃培养24 h。检测各组HepG2细胞甘油三酯(triglyceride,TG)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondialdehyde,MDA)浓度;油红O染色观察各组HepG2细胞的脂滴状态;荧光定量聚合酶链式反应法检测各组HepG2细胞固醇调节元件结合蛋白1(sterol regulatory element binding protein 1,SREBP-1)、二脂酰甘油转移酶2(diacylglyceryltransferase 2,DGAT2)、过氧化物酶体增殖物激活受体α(peroxisomal proliferators activate receptors α,PPARα)、肉毒碱棕榈酰基转移酶1(carnityltransferase 1,CPT1)mRNA相对表达水平。结果:ETOH组细胞发生氧化应激,同时TG含量明显高于正常组、(+)-Cat组和EGCG组;(+)-Cat+ETOH组和EGCG+ETOH组细胞氧化应激反应得到明显改善,同时TG含量显著低于ETOH组(P<0.05,P<0.01),并且显著下调SREBP-1和DGAT2 mRNA表达量(P<0.05,P<0.01),上调PPARα和CPT1 mRNA表达量(P<0.05,P<0.01)。结论:(+)-Cat与EGCG均能改善乙醇诱导的HepG2细胞氧化应激反应和脂代谢紊乱,且EGCG的效果更好。

关键词: (+)-儿茶素;表没食子儿茶素没食子酸酯;脂代谢;氧化应激

Abstract: Objective: To compare in vitro effects of (+)-catechin (Cat) and epigallocatechin gallate (EGCG) on ethanol (ETOH)-induced aberrant lipid metabolism and oxidative stress in HepG2 cells. Methods: HepG2 cells were divided into six groups: normal, 200 μmol/L (+)-Cat, 200 μmol/L EGCG, 300 mmol/L ETOH treatment, 200 μmol/L (+)-Cat plus 300 mmol/L ETOH treatment, and 200 μmol/L EGCG plus 300 mmol/L ETOH treatment. All groups were cultured at 37 ℃ for 24 h. Thereafter, the contents of triglyceride and malondialdehyde (MDA) and superoxide dismutase (SOD) activity were determined. The morphology of lipid droplets in HepG2 cells in each group was observed by Oil Red O staining. The mRNA expression of sterol regulatory element binding protein 1 (SREBP-1), peroxisomal proliferators activate receptors α (PPARα), carnityltransferase 1 (CPT1) and diacylglyceryltransferase 2 (DGAT2) in HepG2 cells were measured by fluorescence quantitative polymerase chain reaction. Results: The level of oxidative stress and TG content in ETOH-treated cells were significantly higher than those in the normal, (+)-Cat and EGCG groups. The oxidative stress response in the (+)-Cat plus ETOH and EGCG plus ETOH groups was significantly improved, and TG content was significantly lower in the two groups than in the ETOH group (P < 0.05, P < 0.01). Moreover, the mRNA expression of SREBP-1 and DGAT2 was down-regulated (P < 0.05, P < 0.01) , and the mRNA expression of PPARα and CPT1 was up-regulated (P < 0.05, P < 0.01). Conclusion: Both (+)-Cat and EGCG can improve ETOH-induced oxidative stress and lipid metabolism disorders in HepG2 cells, and EGCG is more effective.

Key words: (+)-catechin; epigallocatechin gallate; lipid metabolism; oxidative stress

中图分类号: