食品科学 ›› 2021, Vol. 42 ›› Issue (13): 57-63.doi: 10.7506/spkx1002-6630-20200611-152

• 基础研究 • 上一篇    下一篇

响应面法优化辅助降糖降脂的海藻果蔬提取物复配配方

周赛楠,张青,王发合,王晓梅,李兆杰,薛长湖,唐庆娟   

  1. (1.中国海洋大学食品科学与工程学院,山东 青岛 266100;2.青岛明月海藻集团有限公司,海藻活性物质国家重点实验室,山东 青岛 266400;3.青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东 青岛 266237)
  • 出版日期:2021-07-15 发布日期:2021-07-27
  • 基金资助:
    山东省重点研发计划(公益性科技攻关类)项目(2019GHY112058)

Optimization by Response Surface Methodology of the Formulation of Seaweeds, Fruits and Vegetables Extracts to Assist in Reducing Blood Glucose and Lipid Levels

ZHOU Sainan, ZHANG Qing, WANG Fahe, WANG Xiaomei, LI Zhaojie, XUE Changhu, TANG Qingjuan   

  1. (1. College of Food Science and Engineering, Ocean University of China, Qingdao 266100, China;2. State Key Laboratory of Bioactive Seaweed Substances, Qingdao Bright Moon Seaweed Group Co., Ltd., Qingdao 266400, China; 3. Laboratory of Marine Drugs and Biological Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China)
  • Online:2021-07-15 Published:2021-07-27

摘要: 以配方组分对α-葡萄糖苷酶和胰脂肪酶活力的抑制效果——半数抑制浓度(median inhibition concentration,IC50)为指标,通过单因素试验和响应面法研究羊栖菜水提物配比、姜黄素配比和安石榴苷配比对配方抑制α-葡萄糖苷酶和胰脂肪酶活力的影响,优化复配配方。结果表明,海藻果蔬提取物复配配方的最佳配比为m(羊栖菜水提物)∶m(姜黄素)∶m(安石榴苷)=150∶6∶3。实际检测最佳配方的α-葡萄糖苷酶和胰脂肪酶抑制率分别为(47.77±2.15)%和(74.18±0.46)%,与响应面回归方程预测值相近,说明该回归模型能够较好地优化配方。该配方对α-葡萄糖苷酶和胰脂肪酶的IC50分别为(0.56±0.04) mg/mL和(0.210 0±0.000 3)mg/mL,具有良好的辅助降糖降脂的效果。本研究为开发辅助降糖降脂的保健食品提供了科学依据,有助于提高海藻果蔬自然资源的利用率。

关键词: 辅助降糖降脂;海藻;果蔬;复配配方

Abstract: The formulation of Sargassum fusiforme, curcumin (a vegetable bioactive compound) and punicalagin (a fruit bioactive compound) for assisting in lowering blood glucose and lipid levels was optimized using one-factor-at-a-time method and response surface methodology based on median inhibition concentration (IC50) for α-glucosidase and pancreatic lipase. The results showed that the best ratio of the water extract of Sargassum fusiforme to curcumin to punicalagin was 150:6:3 (m/m/m). The inhibition rates of α-glucosidase and pancreatic lipase were (47.77 ± 2.15)% and (74.18 ± 0.46)% by the optimized formulation, respectively, which were close to the predicted values from the response surface regression equation, indicating that the model was reliable. The IC50 values for inhibition of α-glucosidase and pancreatic lipase by the formulation were (0.56 ± 0.04) and (0.210 0 ± 0.000 3) mg/mL, which accordingly could assist in reducing blood glucose and lipid levels. This study provides a scientific basis for the development of dietary supplements that can help reduce blood glucose and lipid levels, which will help improve the utilization rate of natural resources of seaweeds, fruits and vegetables.

Key words: assisting in reducing blood glucose and lipid; seaweeds; fruits and vegetables; formulation

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