食品科学 ›› 2021, Vol. 42 ›› Issue (15): 187-193.doi: 10.7506/spkx1002-6630-20200819-254

• 包装贮运 • 上一篇    下一篇

褪黑素处理对草莓品质与活性氧代谢的影响

黄鸿晖,顾里娟,李美琳,郑永华,金鹏   

  1. (南京农业大学食品科学技术学院,江苏 南京 210095)
  • 出版日期:2021-08-15 发布日期:2021-08-27
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400901)

Effect of Postharvest Melatonin Treatment on Quality and Reactive Oxygen Species Metabolism in Strawberry

HUANG Honghui, GU Lijuan, LI Meilin, ZHENG Yonghua, JIN Peng   

  1. (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2021-08-15 Published:2021-08-27

摘要: 本实验通过研究不同浓度(100、300、500 μmol/L)褪黑素(melatonin,MT)对草莓采后常温贮藏过程中腐烂程度、营养品质的影响,探讨了外源MT处理对‘ 红颜’草莓贮藏期间的保鲜效果。结果表明:3 个不同浓度的MT均能抑制草莓的腐烂,延缓果实硬度和可溶性固形物质量分数的下降,并维持抗坏血酸含量,其中300 μmol/L MT处理的效果最好。此外,还发现300 μmol/L MT在低温(4 ℃)下对草莓贮藏12 d内的抗氧化能力有明显提升:减缓丙二醛和过氧化氢的积累,抑制超氧阴离子自由基生成速率的上升,维持谷胱甘肽与抗坏血酸含量,维持超氧化物歧化酶、过氧化物酶、过氧化氢酶和抗坏血酸过氧化物酶的活性并提高1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子自由基和羟自由基清除率。因此,MT处理可成为延长草莓贮存寿命并维持其品质的有效保鲜方法。

关键词: 草莓;褪黑素;品质;活性氧代谢

Abstract: To evaluate the effect of exogenous melatonin on the quality preservation of strawberry during storage, this work studied the influence of melatonin at different concentrations (100, 300 and 500 μmol/L) on the decay and nutritional quality of strawberry during postharvest storage at 20 ℃. The results showed that melatonin at all concentrations could inhibit fruit decay, delay the decrease of firmness and total soluble solids, and maintain ascorbic acid (ASA) with 300 μmol/L being the most effective concentration. In addition, 300 μmol/L melatonin had a significantly positive effect on the antioxidant capacity of strawberry during storage for up to 12 days at low temperature (4 ℃). It could also slow down the accumulation of malondialdehyde (MDA) and hydrogen peroxide, inhibit superoxide anion radical production, and maintain glutathione (GSH), ascorbic acid content and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX). Moreover, the scavenging capacities of strawberry on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical and hydroxyl radicals were significantly increased after treatment with melatonin. Therefore, melatonin treatment proved to be effective in extending the storage life and maintaining the quality of strawberry.

Key words: strawberry; melatonin; quality; reactive oxygen species metabolism

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