食品科学 ›› 2021, Vol. 42 ›› Issue (18): 212-217.doi: 10.7506/spkx1002-6630-20200901-003

• 成分分析 • 上一篇    下一篇

黑果腺肋花楸果中总花色苷含量测定方法的比较

赵婧,李涵涵,千文,朴昌善,杨长青   

  1. (1.中国药科大学基础医学与临床药学学院,江苏 南京 211198;2.南京珼瑞斯生物医药科技有限公司,江苏 南京 211100;3.延边日鑫生物科技有限公司,吉林 延吉 133000)
  • 发布日期:2021-09-29

Comparison of Method for Determination of Anthocyanins in Black Chokeberry

ZHAO Jing, LI Hanhan, QIAN Wen, PIAO Changshan, YANG Changqing   

  1. (1. School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China; 2. Nanjing BRT-Biomed Co. Ltd., Nanjing 211100, China; 3. Yanbian Ri Xin Biomedical Science and Technology Co. Ltd., Yanji 133000, China)
  • Published:2021-09-29

摘要: 分别探讨摩尔吸光系数和对照品对pH示差法和高效液相色谱(high performance liquid chromatography,HPLC)法测定黑果腺肋花楸果中总花色苷含量的影响,并通过比较优化前后pH示差法和HPLC法的测定结果,确定黑果腺肋花楸果中总花色苷含量的最佳测定方法。采用t检验将优化前后测定方法测得的总花色苷含量进行比较。结果表明,摩尔吸光系数和对照品的选择均会影响测定结果,其中以黑果腺肋花楸果中高含量的矢车菊素-3-O-半乳糖苷为标准的pH示差法和混合标样HPLC法均优于传统方法(以矢车菊素-3-O-葡萄糖苷为标准)(P<0.05);以矢车菊素-3-O-半乳糖苷为标准的单标样HPLC法与混合标样HPLC法测定结果比较无显著差异(P>0.05);HPLC法测定结果均高于pH示差法。结果表明,混合标样HPLC法为测定黑果腺肋花楸果中总花色苷含量最准确的方法,但成本较高;矢车菊素-3-O-半乳糖苷单标样HPLC法简单、准确而经济,同样适用于黑果腺肋花楸果中总花色苷的含量测定。

关键词: 黑果腺肋花楸果;花色苷;高效液相色谱法;pH示差法

Abstract: In this study, the influence of different molar extinction coefficients and reference substances on the determination of total anthocyanins in black chokeberry by the pH differential method and high performance liquid chromatography (HPLC), respectively was investigated. The results obtained by the optimized pH differential method were compared with those obtained by the HPLC method using t-test. Results showed that the selection of molar extinction coefficient and reference substance had a significant impact on the results of the two methods. Moreover, the pH differential method with cyanidin-3-O-galactoside, which is abundant in black chokeberry as the reference substance, and HPLC with a mixed reference standard were superior to the traditional method with cyanidin-3-O-glucoside as the reference substance (P < 0.05). There was no significant difference between the results of HPLC with cyanidin-3-O-galactoside as the reference substance and those with the mixed reference standard (P > 0.05). The results of HPLC were higher than those of the pH differential method. Collectively, despite its low cost, HPLC with the mixed reference standard was the most accurate method for the determination of total anthocyanins in black chokeberry. HPLC with cyanidin-3-O-galactoside as the reference substance was simple, accurate and economical and also suitable for this purpose.

Key words: black chokeberry; anthocyanins; high performance liquid chromatography; pH differential method

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