关键词: 白黄链霉菌；葡萄糖效应；挥发性抑菌物质；1-辛烯-3-醇；黄曲霉；黄曲霉毒素?

Abstract: The growth of Streptomyces alboflavus TD-1 in Gauze’s modified medium with different concentrations of glucose was evaluated by double-dish buckle, and the volatile organic compounds (VOCs) produced by it including 1-octen-3-ol were determined by gas chromatography-mass spectrometry (GC-MS). The antimicrobial mechanism of 1-octen-3-ol against Aspergillus flavus was determined by fluorescence microscopy (FM), scanning electron microscopy (SEM) and high performance liquid chromatography (HPLC). The results showed that the initial growth rate and VOCs production capacity of S. alboflavus TD-1 were severely inhibited by high concentration of glucose (500 mmol/L), but when the glucose concentration was decreased to 50 mmol/L, this inhibition was removed. The antibacterial VOCs produced by S. alboflavus TD-1 included hexanal, 1-octen-3-ol, 2-pentylfuran, and 2-methylisobornyl and acetophenone. Food-grade 1-octen-3-ol completely inhibited the growth of A. flavus at a concentration of 15 μL/L, which had an obvious teratogenic effect on the mycelium and spore morphology of A. flavus, seriously damaged the mitochondrial membrane, and significantly reduced the synthesis rate of AFB1 to 31.98%. Therefore, the concentration of glucose in the medium can affect the growth of S. alboflavus TD-1 and the production of VOCs; 1-octen-3-ol can effectively inhibit the growth of A. flavus and the synthesis of AFB1.

Key words: Streptomyces alboflavus; glucose effect; volatile organic compounds; 1-octen-3-ol; Aspergillus flavus; aflatoxin