食品科学 ›› 2021, Vol. 42 ›› Issue (2): 193-199.doi: 10.7506/spkx1002-6630-20200804-054

• 成分分析 • 上一篇    下一篇

不同品种金针菇特征挥发性物质的差异分析

王鹤潼,潘泓杉,王朝,方东路,胡秋辉,马宁   

  1. (1.南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏省粮油品质控制及深加工技术重点实验室,江苏 南京 210023;2.南京农业大学食品科学技术学院,江苏 南京 210095)
  • 出版日期:2021-01-18 发布日期:2021-01-27
  • 基金资助:
    国家自然科学基金青年科学基金项目(31701660)

Differences in Characteristic Volatile Substances in Different Cultivars of Flammulina filiformis

WANG Hetong, PAN Hongshan, WANG Chao, FANG Donglu, HU Qiuhui, MA Ning   

  1. (1. Key Laboratory of Grains and Oils Control and Processing of Jiangsu Province, Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2021-01-18 Published:2021-01-27

摘要: 采用顶空固相微萃取-气相色谱-质谱(solid phase micro-extraction-gas chromatography-mass spectrometry,SPME-GC-MS)联用技术和电子鼻技术对金针菇5 个品种川金3号、川金11号、川金54号、L4和L7中的挥发性物质进行鉴定并定量分析,并结合主成分分析和聚类分析对金针菇品种归类。结果表明:5 种金针菇中共检测到53 种化合物,8 类挥发性物质,其中醇类、醛类和芳香烃类占总含量的60%~95%。仅鉴定出2 种共有挥发性成分,即1-己醇和2-十一烷酮。电子鼻能将所有品种很好地区分且使各品种样品成团,说明电子鼻数据稳定性和重复性较好,可以辅助SPME-GC-MS数据分析不同品种金针菇挥发性物质。主成分分析和聚类分析都可以将5 个品种有效区分。将SPME-GC-MS和电子鼻结合的多技术鉴别方法可以准确区分金针菇品种,可为不同品种金针菇的选育及后期的加工开发提供一定参考依据。

关键词: 金针菇;品种;固相微萃取-气相色谱-质谱;电子鼻;挥发性成分

Abstract: In this study, by solid phase micro-extraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS) technology and electronic nose (E-nose) technology, we identified and quantitatively analyzed the volatile components in five cultivars of Flammulina filiformis (coded as 3, 11, 54, L4 and L7), and classified the cultivars by principal component analysis (PCA) combined with cluster analysis (CA). The results showed that a total of 53 volatile compounds belonging to eight chemical classes were detected in all cultivars, including alcohols, aldehydes and aromatic hydrocarbons, accounting for 60% to 95% of the total content. Only two volatile substances, 1-hexanol and 2-undecone, were common to all cultivars. E-nose could well distinguish these cultivars and assign samples of each cultivar to one cluster, indicating that the E-nose data are stable and repeatable, and can be used in combination with SPME-GC-MS data to analyze the volatile substances of different cultivars of F. filiformis. In addition, PCA and hierarchical CA distinguished the five cultivars clearly and effectively. This study can provide a reference for the breeding, processing and development of new cultivars of F. filiformis.

Key words: Flammulina filiformis; cultivars; solid phase micro-extraction coupled with gas chromatography-mass spectrometry; electronic nose; volatile components

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