关键词: 金针菇；品种；固相微萃取-气相色谱-质谱；电子鼻；挥发性成分

Abstract: In this study, by solid phase micro-extraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS) technology and electronic nose (E-nose) technology, we identified and quantitatively analyzed the volatile components in five cultivars of Flammulina filiformis (coded as 3, 11, 54, L4 and L7), and classified the cultivars by principal component analysis (PCA) combined with cluster analysis (CA). The results showed that a total of 53 volatile compounds belonging to eight chemical classes were detected in all cultivars, including alcohols, aldehydes and aromatic hydrocarbons, accounting for 60% to 95% of the total content. Only two volatile substances, 1-hexanol and 2-undecone, were common to all cultivars. E-nose could well distinguish these cultivars and assign samples of each cultivar to one cluster, indicating that the E-nose data are stable and repeatable, and can be used in combination with SPME-GC-MS data to analyze the volatile substances of different cultivars of F. filiformis. In addition, PCA and hierarchical CA distinguished the five cultivars clearly and effectively. This study can provide a reference for the breeding, processing and development of new cultivars of F. filiformis.

Key words: Flammulina filiformis; cultivars; solid phase micro-extraction coupled with gas chromatography-mass spectrometry; electronic nose; volatile components