食品科学 ›› 2021, Vol. 42 ›› Issue (22): 140-147.doi: 10.7506/spkx1002-6630-20200815-202

• 生物工程 • 上一篇    

植物乳杆菌XCT 1-10对灰葡萄孢体内和体外生长的影响及其可能机制

李灿婴,程园,侯佳宝,葛永红,杜宏,白凤翎   

  1. (渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁 锦州 121013)
  • 发布日期:2021-11-23
  • 基金资助:
    国家自然科学基金青年科学基金项目(31801595);辽宁省食品科学与工程一流学科专项(LNSPXKBD2020305)

In Vitro and in Vivo Effect of Lactobacillus plantarum XCT 1-10 on the Growth of Botrytis cinerea and Its Possible Mechanisms

LI Canying, CHENG Yuan, HOU Jiabao, GE Yonghong, DU Hong, BAI Fengling   

  1. (National and Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China)
  • Published:2021-11-23

摘要: 研究不同浓度(0、102、104、106、108、1010 CFU/mL)植物乳杆菌(Lactobacillus plantarum)XCT 1-10对灰葡萄孢(Botrytis cinerea)菌落生长、孢子萌发以及接种苹果腐烂的影响。结果表明,106 CFU/mL的L. plantarum XCT 1-10显著抑制了接种B. cinerea的苹果果实病斑扩展,显著抑制了B. cinerea孢子萌发和菌落生长。体外实验表明,L. plantarum XCT 1-10处理提高了B. cinerea培养液中丙二醛、可溶性糖、可溶性蛋白含量和核酸释放,但降低了相对电导率。结果表明,L. plantarum XCT 1-10抑制B. cinerea生长的机制可能与破坏其细胞膜有关。

关键词: 灰葡萄孢;细胞膜;乳酸菌;菌丝生长

Abstract: The effects of Lactobacillus plantarum XCT 1-10 at different concentrations (0, 102, 104, 106, 108, and 1010 CFU/mL) on the colony growth and spore germination of Botrytis cinerea and the decay of apples inoculated with B. cinerea were investigated in this study. The results demonstrated that L. plantarum XCT 1-10 at 106 CFU/mL significantly restrained lesion development in apple fruit inoculated with B. cinerea, and distinctly depressed the spore germination and colony growth of B. cinerea. The in vitro test demonstrated that L. plantarum XCT1-10 treatment increased the contents of malondialdehyde (MDA), soluble sugar and soluble proteins, and nucleic acid release, but decreased the relative conductivity of B. cinerea. These findings suggest that L. plantarum XCT 1-10 can suppress the growth of B. cinerea possibly by destroying the cell membrane.

Key words: Botrytis cinerea; plasma membrane; lactic acid bacteria; mycelial growth

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