食品科学 ›› 2021, Vol. 42 ›› Issue (9): 137-144.doi: 10.7506/spkx1002-6630-2020212-123

• 营养卫生 • 上一篇    下一篇

核黄素结合肽的生物活性及结构表征

阴宏婕,鞠化鹏,钟利敏,孙娜,林松毅   

  1. (1.大连工业大学食品学院,国家海洋食品工程技术研究中心,辽宁 大连 116034;2.赣州市全标生物科技有限公司,江西 赣州 341100)
  • 出版日期:2021-05-15 发布日期:2021-06-02
  • 基金资助:
    江西省重大科技研发专项(20203ABC28W015);2017年度校企合作研究项目(2017210205000075)

Biological Activity and Structural Characterization of Riboflavin-Binding Peptide

YIN Hongjie, JU Huapeng, ZHONG Limin, SUN Na, LIN Songyi   

  1. (1. National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. Ganzhou Quanbiao Biological Technology Co. Ltd., Ganzhou 341100, China)
  • Online:2021-05-15 Published:2021-06-02

摘要: 以松子肽Ac-QWFCT和核黄素为原料制备核黄素结合肽。利用扫描电子显微镜观察其微观特征;通过测定RAW264.7细胞活力、中性红吞噬率和NO释放率评价核黄素结合肽的免疫活性;通过测定1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除率评价核黄素结合肽的抗氧化活性;采用傅里叶变换红外光谱法和核磁共振氢谱法分析肽Ac-QWFCT和核黄素之间的相互作用;利用大鼠翻转肠囊法对核黄素结合肽的吸收特性进行研究。结果表明:与核黄素结合后,肽Ac-QWFCT的微观结构由网状变为絮状;核黄素结合肽的中性红吞噬率和NO释放率均高于肽Ac-QWFCT和核黄素,并在质量浓度25 μg/mL时达到最大值;在质量浓度0.1 mg/mL和0.2 mg/mL时,核黄素结合肽的DPPH自由基和ABTS阳离子自由基清除率明显高于肽Ac-QWFCT和核黄素;肽Ac-QWFCT上的色氨酸和苯丙氨酸残基通过疏水相互作用与核黄素结合;核黄素与肽Ac-QWFCT结合后可以提高核黄素在大鼠肠道的吸收量。本研究可为新型VB2补充剂的开发提供理论依据。

关键词: 松子肽;核黄素;免疫调节活性;抗氧化活性;结构表征

Abstract: The microscopic features of riboflavin-binding peptide, prepared from the pine nut peptide Ac-QWFCT and riboflavin, were examined by scanning electron microscope, and the immunomodulatory activity was evaluated by measuring cell viability, neutral red phagocytosis rate and NO production. Moreover, the antioxidant activity was evaluated by determining the scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals, and Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance were used to determine the interaction between the peptide and riboflavin. The absorption characteristics of riboflavin-binding peptide were studied by the rat everted gut sac method. The results showed that the microstructure of Ac-QWFCT changed from reticular to flocculent after binding with riboflavin. The neutral red phagocytosis rate and NO release rate of riboflavin-binding peptide were higher than those of Ac-QWFCT and riboflavin, reaching the maximum value at a concentration of 25 μg/mL. At 0.1 and 0.2 mg/mL, the DPPH radical and ABTS cation radical scavenging capacities of riboflavin-binding peptide were significantly higher than those of Ac-QWFCT and riboflavin. Tryptophan and phenylalanine residues in the peptide bound to riboflavin through hydrophobic interactions. Binding to the peptide Ac-QWFCT could increase the absorption of riboflavin in the intestine of rats. This study can provide a theoretical basis for the development of new vitamin B2 supplements.

Key words: pine nut peptide; riboflavin; immunomodulatory activity; antioxidant activity; structural characterization

中图分类号: