CRISPR/Cas系统在食源性致病菌快速检测新技术中的研究进展

doi:10.7506/spkx1002-6630-20220316-195

食品科学 ›› 2023, Vol. 44 ›› Issue (7): 313-323.doi: 10.7506/spkx1002-6630-20220316-195

• 专题论述 • 上一篇

CRISPR/Cas系统在食源性致病菌快速检测新技术中的研究进展

寇秀颖，周宝青，陈玲，代京莎，张菊梅，吴清平

（1.无限极（中国）有限公司，广东广州 510405；2.广东省科学院微生物研究所，华南应用微生物国家重点实验室，广东省微生物安全与健康重点实验室，农业农村部农业微生物组学与精准应用重点实验室，广东广州 510070）

发布日期:2023-04-25
基金资助:
“十三五”国家重点研发计划重点专项（2019YFC1606300）；广东省微生物安全与健康重点实验室基金项目（2020B121201009）；广东省科学院创新发展项目（2020GDASYL-20200103024）

Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)

KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping

(1. Infinitus (China) Co., Ltd., Guangzhou 510405, China; 2. State Key Laboratory of Applied Microbiology in Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China)

Published:2023-04-25

摘要/Abstract

摘要： 食源性致病菌的早期筛查与快速检测对食品安全和临床诊断至关重要。然而，传统检测方法操作繁琐、费时费力，难以满足快速检测需求。成簇、规则间隔的短回文重复序列（clustered regularly interspaced short palindromic repeats，CRISPR）和关联蛋白（CRISPR-associated protein，Cas）组成的CRISPR/Cas是广泛存在于细菌和古细菌中的一种免疫系统，其高效特异序列识别及切割活性的特性，为食源性致病菌高灵敏、快速检测提供了一种新的途径。本文介绍了CRISPR/Cas系统的原理、机制及发展，总结了近年来基于CRISPR/Cas系统结合不同结果报告方式用于食源性致病菌快速检测的最新进展，并对其优势、局限性和未来发展方向进行了讨论。

关键词: CRISPR/Cas；食源性致病菌；反式切割；快速检测技术

Abstract: The early screening and rapid detection of foodborne pathogens is critical to food safety and public health. However, the traditional detection method is cumbersome, time-consuming and laborious, and cannot meet the requirement of rapid detection. CRISPR/Cas, composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), is an immune system widely present in bacteria and archaea, which provides a novel pathway for low-cost, rapid, specific and sensitive detection of foodborne pathogens due to its efficient and specific sequence recognition and cleavage activity. This review article introduces the principle, mechanism and development of the CRISPR/Cas system, and summarizes the latest progresses in the rapid detection of foodborne pathogens based on the CRISPR/Cas system combined with different result reporting methods in recent years. Its advantages, limitations and future development prospects are also discussed.

Key words: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein; foodborne pathogens; trans-cleavage; rapid detection technology

中图分类号:

Q93-331

寇秀颖，周宝青，陈玲，代京莎，张菊梅，吴清平. CRISPR/Cas系统在食源性致病菌快速检测新技术中的研究进展[J]. 食品科学, 2023, 44(7): 313-323.

KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping. Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)[J]. FOOD SCIENCE, 2023, 44(7): 313-323.

[1]	张娜娜刘洋俞漪赵渝窦同海徐琼段文锋翁史昱. 乳酸菌饮料中嗜酸乳杆菌的实时荧光定量PCR检测方法[J]. 食品科学, 0, (): 0-0.
[2]	王郅媛，姚婷，范雅为，张燕，王友升. 华莱士瓜采后丝状真菌的分离及rDNA ITS区序列分析[J]. 食品科学, 2018, 39(20): 112-118.
[3]	郗恩光杨劲松. 香蕉植株上乳酸菌的分离鉴定、系统发育分析及抑菌特性[J]. 食品科学, 0, (): 0-0.
[4]	姚婷，黄津津，任向峰，张燕，何欣萌，王友升. 5 株樱桃果实采后病原真菌分离及rDNA ITS区序列分析[J]. 食品科学, 2017, 38(22): 74-79.
[5]	董韩博，谢晶，钱韻芳，杨胜平，苟怡. 抗荧光假单胞菌Carnobacterium spp. LB1培养发酵条件的优化[J]. 食品科学, 2017, 38(10): 55-60.
[6]	黄浩,，郑红发，赵熙，银霞，黄怀生，粟本文，钟兴刚，黄建安，刘仲华,. 不同茶类发花茯茶中“金花”菌的分离、鉴定及产黄曲霉毒素分析[J]. 食品科学, 2017, 38(8): 49-55.
[7]	肖其胜杨捷琳杨惠琴丁卓平袁辰刚韩伟何宇平. 实时荧光PCR法鉴定食品中双歧杆菌[J]. 食品科学, 0, (): 0-0.
[8]	吴慧昊牛锋陈珊珊钟琦. 高效降亚硝酸盐乳酸菌的驯化复筛及分子鉴定[J]. 食品科学, 0, (): 0-0.
[9]	黄科罗洁李泊宁周莎莎庞敏邹勇. 一株生姜Salmonella enterica的分离与宿主氧化应答[J]. 食品科学, 0, (): 0-0.
[10]	何景程楠许文涛. 食品微生物新型快速筛查技术研究进展[J]. 食品科学, 0, (): 0-0.
[11]	李引强吴俊朱宝利张庆波律娜. 16S rRNA的分子生物学方法分析牛奶中的细菌菌群[J]. 食品科学, 0, (): 0-0.
[12]	郭启新. 三种食源性病原菌液相基因芯片检测方法的研究[J]. 食品科学, 0, (): 0-0.
[13]	王泽举，王汝瑱，孙悦，杨莹，刘延琳*. 几株酵母菌的分子系统学鉴定[J]. 食品科学, 2012, 33(15): 195-200.
[14]	宋鹏，陈亮，郭秀璞. 产蛋白酶菌株的鉴定及酶学特性[J]. 食品科学, 2012, 33(13): 152-155.
[15]	曾芹，师俊玲，刘延琳. 产白藜芦醇葡萄内生菌的分离与鉴定[J]. 食品科学, 2012, 33(13): 167-170.

CRISPR/Cas系统在食源性致病菌快速检测新技术中的研究进展

Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价