寒地桑叶多糖化学组成及其生物活性分析

doi:10.7506/spkx1002-6630-20231007-035

食品科学 ›› 2024, Vol. 45 ›› Issue (15): 59-66.doi: 10.7506/spkx1002-6630-20231007-035

寒地桑叶多糖化学组成及其生物活性分析

韩晓云, 郑丹, 张雪瑶, 杜宝昌, 孙庆申

（1.黑龙江大学生命科学学院, 农业微生物技术教育部工程研究中心, 黑龙江省寒区植物基因与生物发酵重点实验室, 黑龙江省普通高校分子生物学重点实验室, 黑龙江省普通高校微生物重点实验室, 黑龙江哈尔滨 150080；2.甘南林场, 黑龙江齐齐哈尔 162100）

出版日期:2024-08-15 发布日期:2024-08-04
基金资助:
黑龙江省自然科学基金联合引导项目（LH2021C075）

Chemical Composition and Biological Activity of Polysaccharides from Mulberry Leaves Grown in Cold Regions

HAN Xiaoyun, ZHENG Dan, ZHANG Xueyao, DU Baochang, SUN Qingshen

(1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region, Key Laboratory of Molecular Biology & Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin 150080, China; 2. Gannan Forest Farm, Qiqihar 162100, China)

Online:2024-08-15 Published:2024-08-04

摘要/Abstract

摘要： 为探究龙桑一号（Morus abla L. cv. Longsang 1）桑叶多糖（mulberry leaf polysaccharide, MLP）的结构及功能性质, 采用水提法提取其MLP, 并对该多糖的结构性质以及体外降糖、抗氧化和免疫活性进行分析。结果表明：提取MLP得率为（16.16±0.30）mg/g, 其中总糖质量分数为（57.16±4.00）%, 糖醛酸质量分数为（30.97±2.06）%。凝胶渗透色谱测得MLP分子质量主要分布在21.74 kDa, 其单糖组成主要包括葡萄糖、鼠李糖、半乳糖、木糖、糖醛酸（包括葡萄糖醛酸、半乳糖醛酸和甘露糖醛酸）、甘露糖, 物质的量比为59.17∶22.31∶10.44∶2.65∶2.28∶1.07。通过傅里叶变换红外光谱和原子力显微镜分析发现MLP是一种吡喃糖, 具有分支和相互交织、螺旋聚集的特点。MLP对α-葡萄糖苷酶和α-淀粉酶抑制作用的半数有效浓度（concentration for 50% of maximal effect, EC50）分别为1.81 mg/mL和2.91 mg/mL, 其活性相当于阿卡波糖的49.17%和37.80%；MLP清除1, 1-二苯基-2-三硝基苯肼自由基、2, 2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基和羟自由基的EC50分别为0.54、0.60 mg/mL和1.77 mg/mL, 清除自由基的效果分别相当于抗坏血酸的4.77%、38.03%和19.94%；MLP可以作用于小鼠巨噬细胞RAW264.7, 使其促炎因子NO、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-1β的分泌量增加, 增强巨噬细胞增殖作用和提高吞噬能力, 从而增强免疫调节作用。本研究结果可为寒地桑叶的应用和功能评价提供实验数据。

关键词: 寒地桑叶多糖；结构特性；免疫活性

Abstract: This study investigated the structure and functional properties including in vitro hypoglycemic, antioxidant, and immunoenhancing activities of water-soluble mulberry leaf polysaccharides (MLP) from Morus abla L. cv. Longsang 1. The results showed that the yield of MLP was (16.16 ± 0.30) mg/g with sugar content of (57.16 ± 4.00)% and uronic acid content of (30.97 ± 2.06)%. The molecular mass distribution of MLP, as measured by gel permeation chromatography (GPC), was mainly concentrated at 21.74 kDa. Monosaccharide composition analysis showed that MLP mainly contained glucose, rhamnose, galactose, xylose, uronic acid (including glucuronic acid, galacturonic acid and mannuronic acid), and mannose, with a molar ratio of 59.17:22.31:10.44:2.65:2.28:1.07. Fourier transform infrared (FT-IR) spectroscopy and atomic force microscopy (AFM) indicated that MLP was a pyranose with intertwined branches and helical aggregation. MLP inhibited α-glucosidase and α-amylase with concentration for 50% of maximal effect (EC50) of 1.81 mg/mL and 2.91 mg/mL, respectively, and its inhibitory activities were 49.17% and 37.80% as high as those of acarbose, respectively. MLP scavenged 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical, and hydroxyl radical with EC50 of 0.54, 0.60 and 1.77 mg/mL, respectively, and the radical scavenging capacities were 4.77%, 38.03%, and 19.94% as high as those of ascorbic acid, respectively. When applied to RAW264.7 mouse macrophages, MLP increased the secretion of the proinflammatory cytokines NO, tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β and enhanced macrophage proliferation and phagocytosis, thereby exerting an immunoenhancing effect. The research results can provide experimental data for the application and functional evaluation of mulberry leaves grown in cold regions.

Key words: polysaccharides from mulberry leave grown in cold regions; structural characteristics; immunological activity

中图分类号:

TS255

韩晓云, 郑丹, 张雪瑶, 杜宝昌, 孙庆申. 寒地桑叶多糖化学组成及其生物活性分析[J]. 食品科学, 2024, 45(15): 59-66.

HAN Xiaoyun, ZHENG Dan, ZHANG Xueyao, DU Baochang, SUN Qingshen. Chemical Composition and Biological Activity of Polysaccharides from Mulberry Leaves Grown in Cold Regions[J]. FOOD SCIENCE, 2024, 45(15): 59-66.

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寒地桑叶多糖化学组成及其生物活性分析

Chemical Composition and Biological Activity of Polysaccharides from Mulberry Leaves Grown in Cold Regions

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