食品科学 ›› 2024, Vol. 45 ›› Issue (16): 121-130.doi: 10.7506/spkx1002-6630-20230726-294

• 成分分析 • 上一篇    下一篇

14 种海棠果实品质分析及综合评价

李文静,刘一心,陈明堃,卓越,吴杨,李厚华   

  1. (西北农林科技大学风景园林艺术学院,陕西 杨凌 712100)
  • 出版日期:2024-08-25 发布日期:2024-08-06
  • 基金资助:
    国家自然科学基金面上项目(32171862);中央高校基本科研业务费专项资金项目(2452023085)

Quality Analysis and Comprehensive Evaluation of 14 Varieties of Crabapples

LI Wenjing, LIU Yixin, CHEN Mingkun, ZHUO Yue, WU Yang, LI Houhua   

  1. (College of Landscape Architecture and Art, Northwest A&F University, Yangling 712100, China)
  • Online:2024-08-25 Published:2024-08-06

摘要: 为全面且直观地评价海棠果实品质,建立海棠果实综合评价体系,选取14 种海棠果实,通过变异性分析、相关性分析及主成分分析筛选品质核心指标,构建海棠果实品质综合评价模型,并结合感官评价、聚类分析验证该模型的可靠性;通过聚类分析对海棠果实进行分类,挖掘不同品种海棠果实的特点及应用价值。结果表明:不同品种海棠果实的18 项品质指标均存在显著差异且显著相关;主成分分析表明5 种主成分累计贡献率为89.253%;结合相关性分析结果可知,单果质量、固酸比、糖酸比和总黄酮含量是评价海棠果实品质的主要指标;海棠果实综合评价模型筛选出‘123小苹果’‘龙丰’‘脆丰’和‘黄太平’4 种品质较佳的海棠果;2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)法、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)法和铁离子还原能力(ferric ion reducing antioxidant power,FRAP)测试结果表明,海棠果实多酚类物质的抗氧化活性与其多酚、黄酮含量呈正向线性拟合,ABTS法得出其拟合系数为R2=0.837和R2=0.728,DPPH法为R2=0.829和R2=0.768,FRAP法为R2=0.575和R2=0.969;海棠果实感官评分与主成分分析得分呈线性拟合,R2=0.862,验证了综合评价模型的可靠性;通过聚类分析对该模型进一步验证,结果显示,海棠果实被分为4 类:‘八棱’‘海红果’为I类,可加工制作高VC含量的保健产品;‘鸡心果’‘龙丰’‘脆丰’‘123小苹果’为II类,口感较好,适宜鲜食;‘亚当’‘丰盛’‘西府’‘白兰地’‘红丽’为III类,适宜制作富含多酚类物质的保健食品;‘K9青沙果’‘凯尔斯’‘黄太平’为IV类,可加工为果脯、果汁、果酒等海棠果产品,该结果与主成分分析得分趋于一致,进一步证实了模型的可靠性。建立海棠果实综合评价体系可为不同品种的海棠果实开发利用提供理论依据,同时为海棠果实品质评价提供优良方案。

关键词: 海棠果实;果实品质;综合评价模型;主成分分析;感官评价;聚类分析

Abstract: A comprehensive and intuitive evaluation system for the quality of crabapples (Malus spp.) was established. In this study, 14 varieties of crabapples were tested. The core quality indexes were selected through variability analysis, correlation analysis, and principal component analysis (PCA) to establish the comprehensive quality evaluation model. The reliability of the model was verified by sensory evaluation and cluster analysis. In addition, the characteristics and utilization values of these varieties were investigated by cluster analysis. The results showed that there were significant differences in 18 quality indexes among the varieties and significant correlations among these quality indexes. PCA showed that the cumulative contribution rate of the first 5 principal components was 89.253%. Combining this result with those of correlation analysis, we found that single fruit mass, solid/acid ratio, sugar/acid ratio, and total flavanol content were the major quality indexes of crabapples. According to the comprehensive evaluation model, ‘123 Xiaopingguo’, ‘Longfeng’, ‘Cuifeng’ and ‘Huangtaiping’ had better fruit quality than the other varieties. The antioxidant activity of crabapple polyphenols showed a positive linear correlation with the contents of polyphenols and flavonoids with correlation coefficients of R2 = 0.837 and 0.728 for 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging capacity, R2 = 0.829 and 0.768 for 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical scavenging capacity, and R2 = 0.575 and 0.969 for ferric ion reducing antioxidant power (FRAP), respectively. Likewise, the sensory evaluation scores were linearly correlated with the PCA scores (R2 = 0.862), indicating the reliability of the comprehensive evaluation model. The results of cluster analysis showed that the crabapple varieties could be divided into 4 categories. Category I included M. orbusta and ‘Haihong’, which could be processed into health products with high VC content. Category II included ‘Jixinguo’, ‘Longfeng’, ‘Cuifeng’ and ‘123 Xiaopingguo’, with good taste and being suitable for fresh consumption. Category III included ‘Adam’, ‘Profusion’, M. micromalus, ‘Brandywine’, and ‘Red Splender’, which are suitable for making polyphenols-rich health foods. Category IV included ‘K9 Qingshaguo’, ‘Kelsey’ and ‘Huangtaiping’, which can be processed into preserved fruit, juice, wine and other products. The results were consistent with those of PCA, corroborating the reliability of the model. The comprehensive quality evaluation system can provide a theoretical basis for the development and utilization of different varieties of crabapples and also provide a good scheme for the quality evaluation of crabapples.

Key words: crabapple; fruit quality; comprehensive evaluation model; principal component analysis; sensory evaluation; cluster analysis

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