鸡蛋过敏人群肠道菌群特征及其与血清细胞因子的相关性

doi:10.7506/spkx1002-6630-20231206-043

食品科学 ›› 2024, Vol. 45 ›› Issue (17): 80-87.doi: 10.7506/spkx1002-6630-20231206-043

鸡蛋过敏人群肠道菌群特征及其与血清细胞因子的相关性

赵童, 杨善平, 陈玲玲, 赵莲英, 刘冬青, 麻微微, 周催

（1.首都医科大学公共卫生学院，北京 100069；2.首都医科大学基础医学院，北京 100069；3.邯郸市第一医院营养科，河北邯郸 056002；4.河北工程大学附属医院激光美容科，河北邯郸 056002；5.邯郸市第一医院检验科，河北邯郸 056002）

出版日期:2024-09-15 发布日期:2024-09-09
基金资助:
国家自然科学基金面上项目（82273633）

Characteristics of Intestinal Flora in Egg-Allergic Population and Their Correlation with Serum Cytokines

ZHAO Tong, YANG Shanping, CHEN Lingling, ZHAO Lianying, LIU Dongqing, MA Weiwei, ZHOU Cui

(1. School of Public Health, Capital Medical University, Beijing 100069, China; 2. School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; 3. Department of Nutriology, Handan First Hospital, Handan 056002, China; 4. Department of Laser Cosmetology, Affiliated Hospital of Hebei Engineering University, Handan 056002, China; 5. Department of Laboratory, Handan First Hospital, Handan 056002, China)

Online:2024-09-15 Published:2024-09-09

摘要/Abstract

摘要： 目的：分析鸡蛋过敏患者肠道菌群组成特征及其与血清细胞因子之间的关系。方法：共招募20 名受试者，采用16S rRNA基因测序方法测定并分析肠道菌群丰度及组成，采用酶联免疫法测定血清中白细胞介素（interleukin，IL）-4、IL-5等食物过敏相关细胞因子的水平；比较两组之间肠道菌群结构与细胞因子水平的差异，并分析两者之间的相关性。结果：与对照组相比，鸡蛋过敏组血清IL-4、IL-5等细胞因子水平显著升高（P＜0.05）。16S rRNA基因测序分析结果显示，β-变形菌目（Betaproteobacteriales）、伯克氏菌科（Burkholderiaceae）和真杆菌属（Eubacterium_hallii_group）等丰度在食物过敏组显著富集（P＜0.05）。采用Spearman相关性分析发现食窦魏斯氏菌（Weissella cibaria）、大肠杆菌属（Escherichia）与血清中细胞因子IL-4、IL-13水平呈负相关。而Burkholderiaceae和uncultured_Parasutterella丰度与转化生长因子β1和IL-13呈正相关。结论：本研究在确定鸡蛋过敏患者特异性肠道菌群，以及在探索肠道菌群在预防或治疗鸡蛋过敏发生方面具有一定参考意义。

关键词: 鸡蛋过敏；肠道菌群；细胞因子；相关性

Abstract: Objective: The present study intended to analyze the characteristics of intestinal floral composition and the relationship with serum cytokines in individuals with egg allergy. Methods: A total of 20 subjects were recruited in this study. The abundance and composition of intestinal flora were determined by 16S rRNA gene sequencing, and the serum levels of food allergy-related cytokines such as interleukin-4 (IL-4) and IL-5 were measured by enzyme immunoassay. The differences in the structure of intestinal flora and the levels of cytokines were compared between the egg allergy and control groups and the correlation between them was analyzed. Results: The serum levels of IL-4 and IL-5 were significantly higher in the egg allergy group compared with the control group (P < 0.05). 16S rRNA gene sequencing showed that Betaproteobacteriales, Burkholderiaceae and Eubacterium_hallii group were significantly enriched in the allergy group (P < 0.05). Spearman’s correlation analysis revealed a negative correlation between the abundance of Weissella cibaria and Escherichia and the serum levels of IL-4 and IL-13. However, the abundance of Burkholderiaceae and uncultured_Parasutterella abundance was positively correlated with transforming growth factor beta1 (TGF-β1) and IL-13. Conclusion: This study provides a scientific basis for determining the specific intestinal flora of egg-allergic individuals and for exploring the implication of intestinal flora in preventing or treating egg allergy.

Key words: egg allergy; intestinal flora; cytokines; correlation

中图分类号:

R593.1

赵童, 杨善平, 陈玲玲, 赵莲英, 刘冬青, 麻微微, 周催. 鸡蛋过敏人群肠道菌群特征及其与血清细胞因子的相关性[J]. 食品科学, 2024, 45(17): 80-87.

ZHAO Tong, YANG Shanping, CHEN Lingling, ZHAO Lianying, LIU Dongqing, MA Weiwei, ZHOU Cui. Characteristics of Intestinal Flora in Egg-Allergic Population and Their Correlation with Serum Cytokines[J]. FOOD SCIENCE, 2024, 45(17): 80-87.

鸡蛋过敏人群肠道菌群特征及其与血清细胞因子的相关性

Characteristics of Intestinal Flora in Egg-Allergic Population and Their Correlation with Serum Cytokines

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