青柠檬腐败真菌分离鉴定及UV结合LED光诱导复合膜液对其抑菌效果

doi:10.7506/spkx1002-6630-20250318-134

食品科学 ›› 2025, Vol. 46 ›› Issue (17): 142-136.doi: 10.7506/spkx1002-6630-20250318-134

• 生物工程 • 上一篇

青柠檬腐败真菌分离鉴定及UV结合LED光诱导复合膜液对其抑菌效果

杨路林，范湘凤，邢亚阁，何丽，但利，雷春晓，田园

（西华大学食品与生物工程学院，四川?成都 610039）

发布日期:2025-08-18
基金资助:
四川省区域创新合作项目（2024YFHZ0207）

Isolation and Identification of Spoilage Fungi from Green Lemons and Their Inhibition by UV-LED-Induced Composite Coating Solution

YANG Lulin, FAN Xiangfeng, XING Yage, HE Li, DAN Li, LEI Chunxiao, TIAN Yuan

(School of Food and Bioengineering, Xihua University, Chengdu 610039, China)

Published:2025-08-18

摘要/Abstract

摘要： 针对重庆潼南地区青柠檬采后贮藏期间易发腐败的问题，从自然腐败的青柠檬中分离纯化出3 株致腐真菌F1、F2和F3。通过形态学观察和分子生物学鉴定，鉴定F1为指状青霉（Penicillium digitatum）、F2为杰克逊青霉（P. jacksonii）、F3为意大利青霉（P. italicum）。随后，采用紫外光（ultraviolet，UV）结合发光二极管（light emitting diode，LED）光诱导壳聚糖（chitosan，CTS）-TiO2-Ag复合膜液进行抑菌实验，评估其对F1～F3致腐真菌的抑菌效果。结果表明，UV结合LED光诱导CTS-TiO2-Ag复合膜液各处理组对F1～F3致腐真菌均有抑菌性，抑菌效果优于单一UV诱导对照组。此外，随着光诱导时间的延长，各处理组抑菌圈直径增大，菌丝干质量减少，菌丝生长抑制率上升，胞外电导率、蛋白质和核酸外渗值增加。总体而言，在相同光诱导时间下，UV结合LED红光处理效果最佳。本研究明确了重庆潼南青柠檬采后腐败的真菌种类，探究了UV结合LED光诱导CTS-TiO2-Ag复合膜液对这些腐败真菌的抑制作用，为开发针对性的柠檬保鲜技术提供了理论支持。

关键词: 青柠檬；腐败真菌；分离鉴定；紫外光；发光二极管；光诱导

Abstract: This study sought to address the problem that green lemons are highly perishable after harvest in Tongnan, Chongqing. Three spoilage fungal strains (F1, F2, and F3) isolated from naturally decayed green lemons were identified by morphological observation and molecular identification as Penicillium digitatum, P. jacksonii, and P. italicum, respectively. Subsequently, the inhibitory effects of ultraviolet (UV)-light emitting diode (LED)-induced chitosan (CTS)-TiO2-Ag composite coating solution against these fungi was evaluated. The results revealed that all UV-LED-induced CTS-TiO2-Ag treatments inhibited each of these strains, and the effect was more pronounced than that of UV-only treatment. Moreover, for each treatment, the diameter of the inhibition zone increased with prolonged light induction, the mycelial dry mass decreased, the inhibition rate of mycelial growth rose, and extracellular electrical conductivity as well as protein and nucleic acid leakage increased. Overall, under the same light induction time, UV-LED red light treatment showed the best fungal inhibitory effect. This study provides theoretical support for developing lemon preservation technology.

Key words: green lemon; spoilage fungi; isolation and identification; ultraviolet; light emitting diode; photoinduction

中图分类号:

Q93-331

杨路林，范湘凤，邢亚阁，何丽，但利，雷春晓，田园. 青柠檬腐败真菌分离鉴定及UV结合LED光诱导复合膜液对其抑菌效果[J]. 食品科学, 2025, 46(17): 142-136.

YANG Lulin, FAN Xiangfeng, XING Yage, HE Li, DAN Li, LEI Chunxiao, TIAN Yuan. Isolation and Identification of Spoilage Fungi from Green Lemons and Their Inhibition by UV-LED-Induced Composite Coating Solution[J]. FOOD SCIENCE, 2025, 46(17): 142-136.

[1]	吕厚姣，李欣媛，白小佳，贾龙刚，耿伟涛，王艳萍. 马乳酒样乳杆菌马乳酒样亚种real-time PCR检测方法的建立与应用[J]. 食品科学, 2024, 45(9): 102-108.
[2]	张娜娜刘洋俞漪赵渝窦同海徐琼段文锋翁史昱. 乳酸菌饮料中嗜酸乳杆菌的实时荧光定量PCR检测方法[J]. 食品科学, 0, (): 0-0.
[3]	王郅媛，姚婷，范雅为，张燕，王友升. 华莱士瓜采后丝状真菌的分离及rDNA ITS区序列分析[J]. 食品科学, 2018, 39(20): 112-118.
[4]	郗恩光杨劲松. 香蕉植株上乳酸菌的分离鉴定、系统发育分析及抑菌特性[J]. 食品科学, 0, (): 0-0.
[5]	姚婷，黄津津，任向峰，张燕，何欣萌，王友升. 5 株樱桃果实采后病原真菌分离及rDNA ITS区序列分析[J]. 食品科学, 2017, 38(22): 74-79.
[6]	董韩博，谢晶，钱韻芳，杨胜平，苟怡. 抗荧光假单胞菌Carnobacterium spp. LB1培养发酵条件的优化[J]. 食品科学, 2017, 38(10): 55-60.
[7]	黄浩,，郑红发，赵熙，银霞，黄怀生，粟本文，钟兴刚，黄建安，刘仲华,. 不同茶类发花茯茶中“金花”菌的分离、鉴定及产黄曲霉毒素分析[J]. 食品科学, 2017, 38(8): 49-55.
[8]	肖其胜杨捷琳杨惠琴丁卓平袁辰刚韩伟何宇平. 实时荧光PCR法鉴定食品中双歧杆菌[J]. 食品科学, 0, (): 0-0.
[9]	吴慧昊牛锋陈珊珊钟琦. 高效降亚硝酸盐乳酸菌的驯化复筛及分子鉴定[J]. 食品科学, 0, (): 0-0.
[10]	黄科罗洁李泊宁周莎莎庞敏邹勇. 一株生姜Salmonella enterica的分离与宿主氧化应答[J]. 食品科学, 0, (): 0-0.
[11]	何景程楠许文涛. 食品微生物新型快速筛查技术研究进展[J]. 食品科学, 0, (): 0-0.
[12]	李引强吴俊朱宝利张庆波律娜. 16S rRNA的分子生物学方法分析牛奶中的细菌菌群[J]. 食品科学, 0, (): 0-0.
[13]	郭启新. 三种食源性病原菌液相基因芯片检测方法的研究[J]. 食品科学, 0, (): 0-0.
[14]	王泽举，王汝瑱，孙悦，杨莹，刘延琳*. 几株酵母菌的分子系统学鉴定[J]. 食品科学, 2012, 33(15): 195-200.
[15]	宋鹏，陈亮，郭秀璞. 产蛋白酶菌株的鉴定及酶学特性[J]. 食品科学, 2012, 33(13): 152-155.

青柠檬腐败真菌分离鉴定及UV结合LED光诱导复合膜液对其抑菌效果

Isolation and Identification of Spoilage Fungi from Green Lemons and Their Inhibition by UV-LED-Induced Composite Coating Solution

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价