普洱茶茶褐素对脂代谢与昼夜节律的调节作用

doi:10.7506/spkx1002-6630-20250921-153

食品科学 ›› 2026, Vol. 47 ›› Issue (8): 195-206.doi: 10.7506/spkx1002-6630-20250921-153

普洱茶茶褐素对脂代谢与昼夜节律的调节作用

郭香，崔宇，严静，赵春燕，雷舒雯，龚加顺，彭春秀

（1.云南农业大学食品科学技术学院，云南昆明 650201；2.云南省农业科学院农产品加工研究所，云南昆明 650233；3.云南农业大学园林园艺学院，云南昆明 650201）

出版日期:2026-04-25 发布日期:2026-05-15
基金资助:
国家自然科学基金地区科学基金项目（82360644）

Pu-erh Tea Theabrownin Regulates Lipid Metabolism and Circadian Rhythm

GUO Xiang, CUI Yu, YAN Jing, ZHAO Chunyan, LEI Shuwen, GONG Jiashun, PENG Chunxiu

(1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2. Agro-products Processing Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650233, China;3. College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China)

Online:2026-04-25 Published:2026-05-15

摘要/Abstract

摘要： 目的：研究普洱茶茶褐素（theabrownin，TB）对脂多糖（lipopolysaccharide，LPS）诱导脂代谢紊乱的调节作用及与代谢相关昼夜节律紊乱的缓解作用。方法：选用SPF级C57BL/6J小鼠，随机分成正常对照组、LPS模型组、低剂量TB组、中剂量TB组和高剂量TB组，干预结束后分别于授时因子时间（zeitgeber time，ZT）0、ZT6、ZT12、ZT18、ZT24处死小鼠。采用常规酶联免疫吸附测定法测定血脂水平、肝脂水平、血清激素水平以及肝损伤指标。通过苏木精-伊红染色、油红O染色检测肝脏纤维化状况恢复、脂滴聚集与积累的情况。采用实时荧光定量聚合酶链式反应和免疫组化法检测与肝脏昼夜节律相关的基因与蛋白表达水平及昼夜节律变化。结果：与正常对照组相比，LPS处理显著增加了小鼠的体质量与脏器指数，显著升高了血清谷丙转氨酶和谷草转氨酶活力。LPS处理伴随着血脂代谢与肝脂代谢紊乱以及昼夜差异规律改变。LPS处理增加了血清内毒素含量并导致了褪黑素分泌节律异常。与LPS处理相比，经TB干预后，上述LPS诱导的病理异常得到了显著改善。进一步分析表明，TB干预改善LPS所致的脂代谢紊乱与调节肝脏时钟基因（即昼夜节律运动输出周期故障蛋白、脑和肌肉芳香烃受体核转录蛋白样1、周期昼夜节律调节蛋白1）和蛋白的表达密切相关。结论：TB可以改善LPS处理引起的小鼠脂代谢紊乱，并进一步缓解LPS处理所致的节律紊乱。

关键词: 普洱茶茶褐素；脂多糖；脂代谢紊乱；昼夜节律；肝脏

Abstract: Objective: This study investigated the regulatory effect of theabrownin (TB) from Pu-erh tea on lipid metabolism disorder induced by lipopolysaccharides (LPS) and its alleviating effect on metabolism-associated circadian rhythm disorders. Methods: SPF C57BL/6J mice were randomly divided into five groups: normal control, LPS model, low-dose TB (LTB), medium-dose TB (MTB), and high-dose TB (HTB). After completion of the intervention, the mice were slaughtered at zeitgeber time (ZT)0, ZT6, ZT12, ZT18, and ZT24. Enzyme-linked immunosorbent assay (ELISA) was used to detect blood lipids, liver lipids, serum hormones and liver injury indicators. The recovery from hepatic fibrosis and lipid droplet aggregation and accumulation were detected by hematoxylin-eosin (HE) staining and red O staining. The expression of genes and proteins linked to liver circadian rhythm were measured using real-time quantitative-polymerase chain reaction (qPCR) and immunohistochemistry (IHC). Results: Compared with the normal control group, LPS treatment significantly increased the body mass and organ indices of mice as well as the activities of serum alanine aminotransferase (ALT) and aspartate transaminase (AST). LPS treatment was accompanied by lipid metabolism disorders and liver lipid metabolism disorders and changes in circadian rhythm. LPS treatment elevated serum endotoxin (ET) levels and caused abnormal secretion rhythm of melatonin (MT). LPS-induced pathological abnormalities were significantly reduced after TB intervention. Further analysis revealed that TB intervention alleviated LPS-induced lipid metabolism disorders, which was closely linked to the regulation of circadian rhythm-related gene and protein expression, such as circadian locomotor output cycle kaput (CLOCK), brain and muscle-Arnt-like protein 1 (BMAL1), and period circadian regulator 1 (PER1). Conclusion: TB can alleviate LPS-induced lipid metabolism disorders and further ameliorate the associated circadian rhythm disturbances in mice.

Key words: Pu-erh tea theabrownin; lipopolysaccharide; lipid metabolism disorders; circadian rhythm; liver

中图分类号:

TS201.4

郭香，崔宇，严静，赵春燕，雷舒雯，龚加顺，彭春秀. 普洱茶茶褐素对脂代谢与昼夜节律的调节作用[J]. 食品科学, 2026, 47(8): 195-206 https://doi.org/10.7506/spkx1002-6630-20250921-153

GUO Xiang, CUI Yu, YAN Jing, ZHAO Chunyan, LEI Shuwen, GONG Jiashun, PENG Chunxiu. Pu-erh Tea Theabrownin Regulates Lipid Metabolism and Circadian Rhythm[J]. FOOD SCIENCE, 2026, 47(8): 195-206 https://doi.org/10.7506/spkx1002-6630-20250921-153

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普洱茶茶褐素对脂代谢与昼夜节律的调节作用

Pu-erh Tea Theabrownin Regulates Lipid Metabolism and Circadian Rhythm

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