食品科学 ›› 2026, Vol. 47 ›› Issue (9): 106-115.doi: 10.7506/spkx1002-6630-20251027-207

• 食品化学 • 上一篇    下一篇

基于糜子糠蛋白的高内相乳液结构调控及其递送应用

李梦卿,江帆,刘洋金,杜双奎   

  1. (1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;2.陕西科技大学食品科学与工程学院,陕西 西安 710021;3.西安市卫生学校,陕西 西安 710054;4.粮油功能化加工陕西省高校工程研究中心,陕西 杨凌 712100)
  • 出版日期:2026-05-15 发布日期:2026-06-03
  • 基金资助:
    陕西省重点研发计划项目(2024NC-YBXM-131);陕西省科技创新团队项目(2024RS-CXTD-70)

Structural Modulation and Application of High Internal Phase Emulsions Containing Proso Millet Bran Protein as a Delivery Carrier

LI Mengqing, JIANG Fan, LIU Yangjin, DU Shuangkui   

  1. (1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;2. School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China; 3. Xi’an Health School, Xi’an 710054, China; 4. Shaanxi Union Research Center of University and Enterprise for Grain Processing Technologies, Yangling 712100, China)
  • Online:2026-05-15 Published:2026-06-03

摘要: 以糜子糠蛋白(proso millet bran protein,PMBP)为乳化剂构建高内相乳液(high internal phase emulsion,HIPE),提升β-胡萝卜素的递送效率,系统评估PMBP质量分数(2%、4%、6%)及水相pH值(5、7、9)对HIPE结构与稳定性的影响。结果表明,随着PMBP质量分数和水相pH值的升高,PMBP-HIPE的粒径减小、Zeta电位绝对值增大,体系在冷藏、冷冻和加热条件下均表现出良好的稳定性。此外,PMBP-HIPE可显著抑制游离脂肪酸释放,并将β-胡萝卜素的体外生物可及性提高至67.47%。其调控机制与PMBP在油水界面形成的致密吸附膜有关,该结构增强静电排斥与空间位阻作用,从而减缓脂质水解并保护活性成分。PMBP-HIPE在脂溶性营养素递送方面具有潜在应用价值,可为在营养输送系统及脂质替代材料中的应用提供理论依据。

关键词: 糜子糠蛋白;高内相乳液;稳定性;递送;体外消化

Abstract: In this study, a high internal phase emulsion (HIPE) stabilized by proso millet bran protein (PMBP) was developed to enhance the delivery of β-carotene. Specifically, the effects of PMBP concentration (2%, 4% and 6%) and aqueous phase pH (5, 7 and 9) on the structure and stability of HIPE were systematically evaluated. As the PMBP concentration or pH increased, the droplet size decreased and the absolute value of the zeta potential increased, indicating improved stability under refrigeration, freezing, and heating conditions. Furthermore, PMBP-stabilized HIPE significantly inhibited the release of free fatty acids and increased the in vitro bio-accessibility of β-carotene to 67.47%. This enhancement was mainly attributed to the dense interfacial adsorption layer formed by PMBP at the oil-water interface, which in turn strengthened both electrostatic repulsion and steric hindrance, thus slowing down lipid hydrolysis and protecting the encapsulated compounds. Overall, PMBP-stabilized HIPE shows strong potential for application in the delivery of lipophilic nutrients, providing a theoretical basis for its application in nutrient delivery systems and lipid alternatives.

Key words: proso millet bran protein; high internal phase emulsion; stability; delivery; in vitro digestion

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