食品科学 ›› 2009, Vol. 30 ›› Issue (12): 61-65.doi: 10.7506/spkx1002-6630-200912008

• 工艺技术 • 上一篇    下一篇

超声波辅助酶法水解玉米蛋白的研究

刘振春,邓子瑜,李 慧,段 绪   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2008-09-22 修回日期:2008-12-29 出版日期:2009-06-15 发布日期:2010-12-29
  • 通讯作者: 刘振春 E-mail:liuzhenchun63@163.com
  • 基金资助:

    吉林省科技厅资助项目(20060220)

Ultrasonic-assisted Enzymatic Hydrolysis of Corn Protein

LIU Zhen-chun,DENG Zi-yu,LI Hui,DUAN Xu   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-09-22 Revised:2008-12-29 Online:2009-06-15 Published:2010-12-29
  • Contact: LIU Zhen-chun E-mail:liuzhenchun63@163.com

摘要:

本实验对玉米蛋白在碱性条件下的酶解工艺进行研究,提出一种超声波预处理和碱性蛋白酶酶解相结合的方法,可在短时间内提高产品得率。通过单因素试验及正交试验的考察,超声波辅助酶法水解玉米蛋白的最佳工艺为:超声波预处理时间4min、底物浓度2%、酶浓度5%、pH10.5、温度55℃。在此条件下,玉米蛋白的水解度为61.47%。

关键词: 玉米蛋白, 水解度, 超声波

Abstract:

The present study aimed to develop a combination method of ultrasonic pre-treatment and alkaline proteasecatalyzed hydrolysis for corn protein. The ultrasonic-assisted enzymatic hydrolysis was optimized through orthogonal test design based on single factor test. When ultrasonic treatment duration, mass fractions of substrate and enzyme, and pH value and temperature for hydrolysis were 4 min, 2% and 5%, and 10.5 and 55 ℃, respectively, the maximum hydrolysis degree was achieved, reaching 61.47%. Moreover, molecular weights of products obtained at different hydrolysis time were determined by SDS-PAGE.

Key words: corn protein, hydrolysis degree, ultrasonic

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