食品科学 ›› 2009, Vol. 30 ›› Issue (10): 219-221.doi: 10.7506/spkx1002-6630-200910050

• 分析检测 • 上一篇    下一篇

茶多酚的流动注射共振光散射法测定

衷明华   

  1. 韩山师范学院
  • 收稿日期:2008-07-27 修回日期:2008-11-17 出版日期:2009-05-15 发布日期:2010-12-29
  • 通讯作者: 衷明华 E-mail:zhongmh@hstc.edu.cn; hszhongmh@126.com

Determination of Tea Polyphones by Flow Injection Resonance Light Scattering Method

ZHONG Ming-hua   

  1. (Department of Chemistry, Hanshan Normal University, Chaozhou 521041, China)
  • Received:2008-07-27 Revised:2008-11-17 Online:2009-05-15 Published:2010-12-29
  • Contact: ZHONG Ming-hua E-mail:zhongmh@hstc.edu.cn; hszhongmh@126.com

摘要:

为了建立测定茶多酚(TP)的流动注射- 共振光散射分析的新方法,在pH7 的NaAc-NH4Ac 缓冲溶液中,通过测定PbTP 微粒的共振散射光强来定量测定TP 的量。在此利用TP 与Pb2+ 生成的沉淀反应,建立了流动注射- 共振光散射联用技术测定TP 的新方法。测定TP 的线性范围为20~400μg/ml,方法的检出限为6.62μg/ml。建立的分析方法简便、试剂少、线性范围宽,可用于茶叶中TP 含量的测定。

关键词: 茶多酚, 流动注射, 共振光散射

Abstract:

To establish a new flow injection resonance light scattering method for the determination of tea polyphones (TP), PbTP was determined by resonance light scattering method in acetic acid buffer medium at pH 7. The amount of TP can be determined directly by mensuring the resonance light scattering intensity of PbTP that is precipitated by reaction of TP with Pb (Ⅱ), and thus a flow injection analysis-resonance light scattering method was proposed to determine TP. The linear range of determination was 20 to 400 μg/ml. The detection limit was 6.62 μg /ml. This method was simple, consumed less reagent and had wide linear range. It can be applied to the determination of TP in tea.

Key words: tea polyphones, flow injection, resonance light scattering

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