关键词: 环十五内酯, 气相色谱法, 外标法

Abstract:

Gas chromatography (GC) with area-external standard method was applied to quantitative analysis of cyclopentadecanolide in its synthesis from Malania oleifera Chun et S.K. Lee kernel oil. A linear regression equation was obtained, which was A = 2238.7C (r = 0.9993), where A was the GC peak area, and C was the cyclopentadecanolide concentration, and the standard curve was validated by single-point external bracketing standard. The recoveries of cyclopentadecanolide in 3.578 mg of sample at the spiked amounts of 1.977, 3.954 and 5.931 mg were 95.11% to 103.3%, the RSDs were 1.51% to 2.19% (n = 3). The determination results of cyclopentadecanolide content in four prepared sample solutions were 2.14 to 4.52 mg/ml, and the RSDs were 0.66% to 2.36% (n = 3).

Key words: cyclopentadecanolide, gas chromatography, area-external standard method