食品科学 ›› 2009, Vol. 30 ›› Issue (6): 235-237.doi: 10.7506/spkx1002-6630-200906055

• 分析检测 • 上一篇    下一篇

细脚拟青霉深层发酵菌丝体核苷类成分分析

陈安徽1,邵 颖1,樊美珍2,吴海亮2   

  1. 1.徐州工程学院食品(生物)工程学院 2.安徽省微生物防治重点实验室
  • 收稿日期:2008-04-07 修回日期:2008-08-28 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 陈安徽 E-mail:chenah201@163.com
  • 基金资助:

    国家自然科学基金资助项目(30570004;30570189)

Analysis of Nucleosides in Mycelia of Paecilomyces tenuipes

CHEN An-hui1,SHAO Ying1,FAN Mei-zhen2,WU Hai-lian   

  1. (1. Food Engineering Department, Xuzhou Institute of Technology, Xuzhou 221008, China;
    2. Anhui Provincial Key Laboratory of Microbial Pest Control, Hefei 230036, China)
  • Received:2008-04-07 Revised:2008-08-28 Online:2009-03-15 Published:2010-12-29
  • Contact: CHEN An-hui1 E-mail:chenah201@163.com

摘要:

以已知核苷为标样,并以冬虫夏草生药作对比,利用反相高效液相色谱法对细脚拟青霉RCEF0441 深层发酵菌丝体中核苷类化学成分进行分析。结果表明,细脚拟青霉RCEF0441 菌丝体中主要含有胞苷、尿苷、鸟苷、肌苷、胸苷和腺苷6 种核苷,其中胞苷的含量为冬虫夏草生药的3 倍左右,肌苷和鸟苷的含量则分别是冬虫夏草生药的7 和9 倍左右,尿苷和腺苷的含量也显著高于对照品冬虫夏草生药(p<0.05)。

关键词: 细脚拟青霉, 菌丝体, 核苷

Abstract:

The nucleoside compositions in Cordyceps sinensis and fermentation mycelia of Paecilomyces tenuipes RCEF0441 were determined by high performance liquid chromatography (HPLC). The results showed that the nucleosides in mycelia of Paecilomyces tenuipes RCEF0441 include mainly adenosine, uridine, guanosine, inosine, cytidine and thymidine. The contents of cytidine, inosine and guanosine are about 3, 7 and 9 times of those in Cordyceps sinensis, respectively. Moreover, the contents of adenosine and uridine are obviously higher than those in Cordyceps sinensis.

Key words: Paecilomyces tenuipes, mycelia, nucleosides

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