关键词: 虾夷扇贝；游离氨基酸；5’-核苷酸；脂质

Abstract: In order to reveal the differences in the nutritional value and flavor of different genders and tissues of scallop (Patinopecten yessoensis), free amino acids, 5’-nucleotides and lipids in the samples were analyzed by targeted and non-targeted metabolomics based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and ultra-high performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). Results showed that glutamic acid and glycine were the main free amino acids in scallop. The contents of glutamic acid and glycine in the adductor muscle were 132.98 and 280.37 μg/g, respectively and were little affected by gender. Adenosine 5’-monophosphate (AMP) was abundant in the adductor muscle and mantle, and the content of AMP in the adductor muscle of female scallops was about twice higher than that in male scallops. A total of 188 lipids were detected in the tissues of scallop, including phospholipids and glycerides. The content of phospholipids in the adductor muscle of male scallops was higher than that in female scallops, while the opposite was true for the content of phospholipids in the gonads. The digestive gland was an important part for storing glycerides, accounting for 49.36% and 33.11% of the total glycerides in male and female scallops, respectively. These results are of great significance for guiding the development of scallop products and the comprehensive processing and utilization of scallop by-products.

Key words: Patinopecten yessoensis; free amino acids; 5’-nucleotides; lipids