食品科学 ›› 2010, Vol. 31 ›› Issue (15 ): 216-220.doi: 10.7506/spkx1002-6630-201015047

• 生物工程 • 上一篇    下一篇

米根霉L- 乳酸发酵菌丝球形成条件的研究

蒋雪薇1 , 2,罗晓明2,盛灿梅2,高必达1 ,*   

  1. 1.湖南农业大学生物安全科学技术学院 2.长沙理工大学化学与生物工程学院
  • 收稿日期:2010-05-07 出版日期:2010-08-15 发布日期:2010-12-29
  • 通讯作者: 高必达 E-mail:bdgao@yahoo.com.cn
  • 基金资助:

    湖南省教育厅资助项目(06C090);微生物分子生物学湖南省重点实验室开放基金项目(MM0802)

Effects of Culture Conditions of Mycelial Pellet Formation and L-Lactic Acid Production by Rhizopus oryzae

JIANG Xue-wei1,2,LUO Xiao-ming2,SHENG Can-mei2,GAO Bi-da1,*   

  1. 1. College of Bio-safety Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, China
  • Received:2010-05-07 Online:2010-08-15 Published:2010-12-29
  • Contact: GAO Bi-da1 E-mail:bdgao@yahoo.com.cn

摘要:

研究米根霉CS323-9 在摇瓶培养条件下影响菌丝成球及产乳酸的因素。考察(NH4)2SO4 质量浓度、接种量、摇瓶转速及表面活性剂吐温-80 用量4 个因素,再利用L9(34)的正交试验,对菌丝球形成及产酸的条件进行优化。结果表明:(NH4)2SO4 质量浓度4g/L、接种量7%、摇瓶转速200r/min、吐温-80 用量0.5g/L 时,摇瓶发酵菌丝球数目达115 个/mL、菌丝球平均直径为1.2mm、L- 乳酸积累量为78.9g/L。说明米根霉CS323-9 在摇瓶培养条件下菌丝球的形成与乳酸的积累有较大的相关性。

关键词: 米根霉, L - 乳酸, 菌丝球, 正交试验

Abstract:

The effects of (NH4)2SO4 concentration, inoculum size, shake flask agitation speed and quantity of Tween-80 on the growht of Rhizopus oryzae mycelial pellets for L-lactic acid production were studied using shake flasks. Based on orthogonal array optimization, ammonium sulfate concentration of 4 g/L, inoculum size of 7% (V/V), shake flask agitation speed of 200 r/min and quantity of Tween-80 of 0.5 g/L were found optimum. The quantity of mycelial pelletswas up to 115 per milliliter after culture for 48 under these conditions, the average pellet diameter 1.2 mm and the yield of L-lactic acid 78.9 g/L. Our findings suggest that the grwoth of Rhizopus oryzae mycelial pellets is largely correlated with L-lactic acid production.

Key words: Rhizopus oryzae, L-lactic acid, mycelial pellets, orthogonal array design

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