食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 238-241.doi: 10.7506/spkx1002-6630-200924050

• 工艺技术 • 上一篇    下一篇

鱿鱼眼透明质酸降解工艺条件研究

王 菁,杨佳玉,金 淼,马金莲,卢佳芳,杨文鸽*   

  1. 宁波大学生命科学与生物工程学院,应用海洋生物技术教育部重点实验室
  • 收稿日期:2009-08-15 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 杨文鸽* E-mail:yangwene@nbu.edu.cn
  • 基金资助:

    农业部“948”项目(2006G-41);浙江省新苗人才计划项目(2008R40G2070044)

Degradation Condition of Hyaluronic Acid from Squid Eyes

WANG Jing,YANG Jia-yu,JIN Miao,MA Jin-lian,LU Jia-fang,YANG Wen-ge*   

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Faculty of Life Science and Biotechnology,
    Ningbo University, Ningbo 315211, China
  • Received:2009-08-15 Online:2009-12-15 Published:2010-12-29
  • Contact: YANG Wen-ge* E-mail:yangwene@nbu.edu.cn

摘要:

以鱿鱼眼中提取的透明质酸为原料,以相对分子质量为指标,选取物理和化学方法降解透明质酸,研究不同降解工艺对产物相对分子质量的影响,得到了透明质酸降解的最适条件:采用H2O2 和抗坏血酸结合的方法降解透明质酸,H2O2 和抗坏血酸的最佳物质的量的比为3:1、pH4.0、反应时间15min、反应温度50℃,透明质酸的相对分子质量可降解到5433。

关键词: 鱿鱼眼, 透明质酸, 降解, 相对分子质量

Abstract:

Hyaluronic acid (HA) is a unique acidic mucopolysaccharide with multiple physiological functions so that it is widely applied in the fields of medicine and food due to its various molecular weights. Squid eyes were used as raw materials to study degradation condition of HA through physical and chemical methods. Molecular weight was used as the index to evaluate degradation condition. Results indicated that HA could be effectively degraded by a combinatorial method of hydrogen peroxide and ascorbic acid. The optimal degradation condition was 3:1 of mole ratio between hydrogen peroxide and ascorbic acid, pH 4.0, 15 min of reaction time, 50 ℃ of reaction temperature. The molecular weight of HA could be degraded to 5433 under this optimal condition.

Key words: quid eyes, hyaluronic acid, degradation, molecular weight

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