食品科学 ›› 2009, Vol. 30 ›› Issue (23 ): 365-369.doi: 10.7506/spkx1002-6300-200923083

• 生物工程 • 上一篇    下一篇

植物乳杆菌F22 对黄曲霉毒素B1 的吸附特性

曾 东1,2,唐雨蕊1,倪学勤1,2,*,张卓立1,冉 君1   

  1. 1.四川农业大学动物医学院 2.动物疫病与人类健康四川省重点实验室
  • 收稿日期:2009-08-15 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 倪学勤 E-mail:xueqinni@yahoo.com
  • 基金资助:

    教育部“长江学者和创新团队发展计划”项目(IRT0555;IRT0848);四川省科技厅国际合作项目(2008HH0016)

Absorption Characteristics of Lactobacillus plantarum F22 to Aflatoxin B1

ZENG Dong1,2,TANG Yu-rui1,NI Xue-qin1,2,*,ZHANG Zhuo-li1,RAN Jun1   

  1. 1. College of Veterinary, Sichuan Agricultural University, Ya'an 625014, China;2. Key Laboratory of Animal Disease and Human
    Health of Sichuan Province, Ya'an 625014, China
  • Received:2009-08-15 Online:2009-12-01 Published:2010-12-29
  • Contact: NI Xue-qin E-mail:xueqinni@yahoo.com

摘要:

采用体外筛选法从14 株乳酸杆菌中筛选能吸附黄曲霉毒素B1(AFB1)的菌株,并探讨其吸附特性和机理。结果表明,植物乳杆菌F22 吸附率最高;F22 处理AFB1 30min 的吸附率较处理4h 高(P < 0.01);热处理使吸附率极显著上升(P < 0.01);而人工胃肠液处理则导致吸附率显著下降(P < 0.05);冻干过程对F22 吸附率的影响不显著(P > 0.05)。乳酸杆菌对AFB1 的吸附具有菌株特异性,吸附效果与细菌细胞壁的组成和结构相关。

关键词: 乳酸杆菌, 黄曲霉毒素B1, 筛选, 吸附

Abstract:

In order to study absorption characteristics and mechanisms of Lactobacillus plantarum to aflatoxin B1 (AFB1), 14 strains with absorption capability to AFB1 were screened in vitro. Lactobacillus plantarum F22 exhibited the highest absorption to AFB1. Effects of F22 under various incubation times, heating-treatment, mimic gastric intestinal environment treatment, and lyophilization processing on absorption rate to AFB1 were also investigated. Incubation of F22 and AFB1 for 30 min showed the highest absorption (P<0.01). Heating-treatment promoted absorption capability to AFB1 (P<0.01). In contrast, mimic gastric intestinal environment caused a significant decrease in absorption to AFB1 (P<0.05) and no significant effect on absorption to AFB1 was observed during lyophilization processing (P>0.05). Absorption capability of Lactobacillus plantarum to AFB1 was strain-dependent due to the composition and structure of cell wall.

Key words: Lactobacillus, AFB1, screening, absorption

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