食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 188-191.doi: 10.7506/spkx1002-6300-200921044

• 生物工程 • 上一篇    下一篇

紫外线与亚硝基胍复合诱变选育高产海藻糖菌株的研究

张春玲   

  1. 东北电力大学输变电技术学院
  • 收稿日期:2009-06-13 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 张春玲 E-mail:jlzcl@126.com

UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae

ZHANG Chun-ling   

  1. College of Power Transmission and Transformation on Technology, Northeast Dianli University, Jilin 132012, China
  • Received:2009-06-13 Online:2009-11-01 Published:2010-12-29
  • Contact: ZHANG Chun-ling E-mail:jlzcl@126.com

摘要:

本实验以酿酒酵母为出发菌株,用紫外线与亚硝基胍复合诱变选育出一株遗传稳定性较好的高产海藻糖菌株,其海藻糖含量为19.8%(以干酵母计),比原菌株提高了39.43%,比只用紫外线诱变后的菌株提高了10.0%,比只用亚硝基胍诱变后的菌株提高了6.45%。

关键词: 紫外线, 亚硝基胍, 海藻糖, 复合诱变, 酿酒酵母

Abstract:

A high trehalose-producing strain of Saccharomyces cerevisiae was bred via combined nitrosoguanidine (NTG)/UV mutagenesis. This strain had good genetic stability and a trehalose content of 19.8% (m/m dry yeast), which revealed the enhancement of 39.43%, 10.0% and 6.45% compared with the original strain, the strain screened by alone UV mutagenesis, and the strain screened by alone NTG mutagenesis, respectively.

Key words: UV, NTG, trehalose, combined mutagenesis, Saccharomyces cerevisiae

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