食品科学 ›› 2009, Vol. 30 ›› Issue (16 ): 135-136.doi: 10.7506/spkx1002-6630-200916025

• 工艺技术 • 上一篇    下一篇

茜草多糖微波提取工艺及含量测定研究

田春莲1,向 斌2   

  1. 1.吉首大学生物资源与环境科学学院 2.吉首大学城乡资源与规划学院
  • 收稿日期:2009-06-11 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 田春莲 E-mail:tianchunlian1970@163.com

Microwave Extraction and Content Determination of Polysaccharides from Rubia cordifolia L.

,XIANG Bin2   

  1. 1. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;
    2. College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China
  • Received:2009-06-11 Online:2009-08-15 Published:2010-12-29
  • Contact: TIAN Chun-lian E-mail:tianchunlian1970@163.com

摘要:

利用正交试验确定茜草多糖的最佳提取工艺,运用蒽酮- 硫酸法测定茜草不同营养器官的多糖含量。结果表明:用水作溶剂,料液比1:40,微波中等火力提取60s,提取效率较好,多糖提取率高达10.20%;茜草不同营养器官的多糖含量以老叶最高为13.34%,其次是老茎10.28%。

关键词: 茜草多糖, 微波提取, 蒽酮- 硫酸法

Abstract:

The microwave extraction of polysaccharides from Rubia cordifolia L. was optimized to attain the maximum extraction yield by orthogonal array design, and the contents of polysaccharides in different parts of Rubia cordifolia L. were measured by anthrone-sulfuric acid method. The medium-fire microwave extraction for 60 s with the ratio of material to water of 1:40 led to the highest extraction yield of 10.20%. Old leaves of Rubia cordifolia L. had the highest content of polysaccharides of 13.34%, followed by old stems, 10.28%.

Key words: Rubia cordifolia L. polysaccharides, microwave extraction, anthrone-sulfuric acid method

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