食品科学 ›› 2011, Vol. 32 ›› Issue (22): 32-36.doi: 10.7506/spkx1002-6630-201122007

• 工艺技术 • 上一篇    下一篇

响应面法优化刺槐花黄酮类化合物的微波提取工艺

向昌国1,向 宁2,黄成龙3,孙海滨3,李文芳3   

  1. 1.吉首大学 生态旅游应用技术湖南省重点实验室 2.湖南农业大学食品科技学院 3.吉首大学城乡资源与规划学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    生态旅游应用技术湖南省重点实验室资助项目(JDSTLY201106)

Optimization of Microwave Extraction of Flavonoids from Robinia pseudoacacia L. Flowers Using Response Surface Methodology

XIANG Chang-guo1,XIANG Ning2,HUANG Cheng-long3,SUN Hai-bin3,LI Wen-fang3   

  1. (1. Hunan Application Technology of Ecotourism Key Laboratory, Jishou University, Zhangjiajie 427000, China; 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 目的:利用响应面法对刺槐花黄酮类化合物的提取工艺进行优化。方法:在单因素试验的基础上,选择液料比、乙醇体积分数、微波时间为自变量,黄酮类化合物提取量为响应值,利用响应面分析法,研究各自变量交互作用及其对黄酮类化合物提取量的影响,模拟得到二次多项式回归方程的预测模型。结果:刺槐花黄酮类化合物的提取工艺为乙醇体积分数75%、液料比37:1(mL/g)、微波时间145s、提取两次。结论:在最佳工艺条件下黄酮类化合物提取量为8.63mg/g。

关键词: 刺槐花, 黄酮类化合物, 响应面法, 微波提取

Abstract: The optimum conditions for the extraction of flavonoids from Robinia pseudoacacia L. flowers were determined using response surface methodology. On the basis of one-factor-at-a-time experiments, Box-Benhnken central composite design was applied to investigate the effects of three variables, namely alcohol concentration, liquid-to-solid ratio and microwave heating time on extraction yield of flavonoids. A regression model describing extraction yield of flavonoids as a function of these three variables was established. The best results of the extraction of flavonoids from Robinia pseudoacacia L. flowers were obtained when the samples were extracted twice by means of microwave heating for 145 s at a ratio of liquid to solid of 37:1 using 75% ethanol. Under these conditions, the extraction yield of flavonoids was 8.63 mg/g.

Key words: Robinia pseudoacacia L. flowers, flavonoids, response surface methodology, microwave-assisted extraction

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