食品科学 ›› 2009, Vol. 30 ›› Issue (15 ): 120-123.doi: 10.7506/spkx1002-6630-200915026

• 基础研究 • 上一篇    下一篇

棘托竹荪子实体抑菌活性的研究

卢惠妮,潘迎捷,孙晓红,赵 勇*   

  1. 上海海洋大学食品学院
  • 收稿日期:2009-05-13 出版日期:2009-08-01 发布日期:2010-12-29
  • 通讯作者: 赵 勇* E-mail:yzhao@shou.edu.cn
  • 基金资助:

    霍英东教育基金会第十一届高等院校优选资助课题(114035)

Antibacterial Activity of Water Extract of Dictyophora echinovolvata Fruitbody

LU Hui-ni,PAN Ying-jie,SUN Xiao-hong,ZHAO Yong*   

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Received:2009-05-13 Online:2009-08-01 Published:2010-12-29
  • Contact: ZHAO Yong* E-mail:yzhao@shou.edu.cn

摘要:

以水为提取剂,对棘托竹荪子实体进行浸提,利用琼脂平板打孔法测定棘托竹荪子实体对大肠杆菌O157:H7、副溶血弧菌、单增李斯特菌、肠炎沙门氏菌和金黄色葡萄球菌的抑菌效果,并研究其对5 种病原菌的最低抑菌浓度(MIC)、热稳定性以及抑菌pH 值范围。结果表明,棘托竹荪子实体的浸提液对副溶血弧菌抑制作用最好,其次是单增李斯特菌、大肠杆菌O157,对肠炎沙门氏菌、金黄色葡萄球菌抑制效果最差;棘托竹荪子实体的浸提液最低抑菌浓度小,抑菌pH 值范围广而且热稳定性强。

关键词: 棘托竹荪, 子实体, 抑菌作用, 最低抑菌浓度

Abstract:

Aqueous extract of Dictyophora echinovolvata fruitbody was prepared by boiling water extraction and its antibacterial activity against 5 species of common food-borne pathogenic bacteria was investigated by agar diffusion plate method. Additionally, the minimum inhibitory concentration (MIC) and thermal stability as well as antibacterial pH range of this extract were also analyzed. The extract of Dictyophora echinovolvata fruitbody displayed the highest inhibitory effect against Vibrio parahaemolyticus, followed by Listeria monocytogenes and Escherichia coli O157:H7 while no significant bacteriostatic effects were observed on Salmonella enteritidis and Staphylococcus aureus. This extract possessed quite lower MIC values, wide antibacterial pH range and the strong thermal stability.

Key words: Dictyophora echinovolvata, fruitbody, bacteriostatic effect, minimum inhibitory concentration (MIC)

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