食品科学

• 基础研究 • 上一篇    下一篇

6种食用菌子实体水提物对肠道菌群的影响

赵睿秋1,马高兴2,杨文建1,裴 斐1,赵立艳2,胡秋辉1,*   

  1. 1.南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,江苏 南京 210023;2.南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2017-03-15 发布日期:2017-03-28

Effects of Water Extracts from Six Different Species of Edible Mushroom on Intestinal Microorganisms in Humans

ZHAO Ruiqiu1, MA Gaoxing2, YANG Wenjian1, PEI Fei1, ZHAO Liyan2, HU Qiuhui1,*   

  1. 1. Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China; 2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2017-03-15 Published:2017-03-28

摘要: 以金针菇、香菇、黑木耳、平菇、双孢菇、杏鲍菇6 种常见的市售食用菌子实体为研究对象,利用Illumina MiSeq PE250二代测序平台对肠道微生物V4区进行富集测序,分析6 种食用菌子实体水提物对粪便悬液中肠道菌群的菌群种类和菌群结构的影响。测序结果显示经过不同实验组培养后,不同种类的食用菌子实体对肠道微生物的影响各不相同,金针菇组的厚壁菌门水平较高;香菇组的拟杆菌门、变形菌门、厚壁菌门、双歧杆菌和乳杆菌水平较高;黑木耳组的拟杆菌门、放线菌门、厚壁菌门、双歧杆菌和乳杆菌水平较高;平菇组的拟杆菌门、放线菌门、厚壁菌门和乳杆菌水平较高;双孢菇组的拟杆菌门、放线菌门水平较高;杏鲍菇组的放线菌门水平较高。

关键词: 食用菌子实体, 肠道微生物, 厌氧培养, 高通量测序技术

Abstract: This study researched the effects of water extracts from six different species of edible mushroom, Flammulina velutipes, Lentinus edodes, Auricularia auricula, Pleurotus ostreatus, Agaricus bisporus and Pleurotus eryngii, on the microbial community composition and structure in the human intestine. Towards this goal, MiSeq PE250 Illumina nextgeneration sequencing platform was used to identify the sequence of the 16S rDNA V4 region from the bacterial cells. Sequencing results showed that different species of edible mushrooms had different impacts on the intestinal mictrobiota. Higher populations of Firmicutes were found in the Flammulina velutipes group, Bacteroidetes, Proteobacteria, Firmicutes, Bifidobacterium and Lactobacillus in the Lentinus edodes group, Bacteroidetes, Actinomycetes, Firmicutes, Bifidobacterium and Lactobacillus in the Auricularia auricular group, Bacteroidetes, Actinomycetes, Firmicutes and Lactobacillus in the Pleurotus ostreatus group, Bacteroidetes and Actinomycetes in the Agaricus bisporus group, and Actinomycetes in the Pleurotus eryngii group.

Key words: edible mushroom, intestinal microorganism, anaerobic culture, high throughput sequencing technology (HTS)

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