食品科学 ›› 2008, Vol. 29 ›› Issue (4): 154-157.
• 工艺技术 • 上一篇 下一篇
杨巍, 陈庆森, 符浩, 金美玲
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YANG Wei, CHEN Qing-Sen, FU Hao, JIN Mei-Ling
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摘要: 本实验从牛初乳中分离纯化胰岛素样生长因子-Ⅰ(IGF-Ⅰ),重点对分离获取高水平IGF-I的前处理条件进行了较为细致的条件优化,每一步骤中IGF-Ⅰ的质量浓度检测均采用放射免疫法测定。为后续工作中采用超滤法规模化、高水平生产IGF-Ⅰ提供了实验依据。
关键词: 牛初乳, 胰岛素样生长因子-Ⅰ(IGF-I), 前处理
Abstract: In this study, the insulin-like growth factor -Ⅰ(IGF-Ⅰ) in bovine colostrums was separated and purified. Especially, the study focused on the optimization of pretreatment conditions in the separation to access high level of IGF-Ⅰ. The content of IGF-Ⅰ in each process was detected by radio-immunoassay (RIA). Provide the basis for follow-up work to further purified with scale ultra-filtration and high-level production of IGF-I in the future.
Key words: colostrums, insulin- like growth factor-Ⅰ(IGF-I), pretreatment
杨巍, 陈庆森, 符浩, 金美玲. 牛初乳中IGF-Ⅰ分离纯化过程中前处理条件的优化研究[J]. 食品科学, 2008, 29(4): 154-157.
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