食品科学 ›› 2008, Vol. 29 ›› Issue (4): 154-157.

• 工艺技术 • 上一篇    下一篇

牛初乳中IGF-Ⅰ分离纯化过程中前处理条件的优化研究

杨巍, 陈庆森, 符浩, 金美玲   

  1. 天津市食品生物技术重点实验室 天津商业大学生物技术与食品科学学院; 天津市食品生物技术重点实验室; 天津商业大学生物技术与食品科学学院;
  • 出版日期:2008-04-15 发布日期:2011-08-24

Optimal of Pretreatment Conditions in Separated and Purified of Insulin-like Growth Factor-Ⅰ in Bovine Colostrums

 YANG  Wei, CHEN  Qing-Sen, FU  Hao, JIN  Mei-Ling   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce
  • Online:2008-04-15 Published:2011-08-24

摘要: 本实验从牛初乳中分离纯化胰岛素样生长因子-Ⅰ(IGF-Ⅰ),重点对分离获取高水平IGF-I的前处理条件进行了较为细致的条件优化,每一步骤中IGF-Ⅰ的质量浓度检测均采用放射免疫法测定。为后续工作中采用超滤法规模化、高水平生产IGF-Ⅰ提供了实验依据。

关键词: 牛初乳, 胰岛素样生长因子-Ⅰ(IGF-I), 前处理

Abstract: In this study, the insulin-like growth factor -Ⅰ(IGF-Ⅰ) in bovine colostrums was separated and purified. Especially, the study focused on the optimization of pretreatment conditions in the separation to access high level of IGF-Ⅰ. The content of IGF-Ⅰ in each process was detected by radio-immunoassay (RIA). Provide the basis for follow-up work to further purified with scale ultra-filtration and high-level production of IGF-I in the future.

Key words: colostrums, insulin- like growth factor-Ⅰ(IGF-I), pretreatment