食品科学 ›› 2008, Vol. 29 ›› Issue (3): 176-180.

• 工艺技术 • 上一篇    下一篇

响应曲面法优选灵芝子实体多糖提取工艺(英文)

 宋江峰, 刘春泉, 李大婧, 金邦荃   

  1.  南京师范大学金陵女子学院; 江苏省农业科学院原子能农业利用研究所; 南京师范大学金陵女子学院 江苏南京210097 江苏省农业科学院原子能农业利用研究所; 江苏南京210014; 江苏南京210097;
  • 出版日期:2008-03-15 发布日期:2011-08-24

Optimization of Extraction Process of Ganoderma lucidum Fruitbody Polysaccharides by Response Surface Methodology

 SONG  Jiang-Feng, LIU  Chun-Quan, LI  Da-Jing, JIN  Bang-Quan   

  1. 1.Jinling College, Nanjing Normal University, Nanjing 210097, China 2.Institute of Atomic Energy in Application for Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
  • Online:2008-03-15 Published:2011-08-24

摘要: 本研究采用响应曲面法对影响GLP提取率的三个主要影响因素即提取温度、提取时间和液固比进行了优化。利用Design Expert软件对GLP提取率的二次多项数学模型分析表明:在提取温度为89.35℃,提取时间1.47h,液固比29.19:1时,GLP提取率较高,最佳提取率预测值为0.776%,与实测值基本相符。利用优化工艺参数提取GLP时,可获得最大的提取率。

关键词: GLP, 响应曲面法, 提取率

Abstract: The effects of different temperature, time and the ratio of solid to solution on the extraction yield of Ganoderma lucidum polysaccharides (GLP) were optimized with response surface methodology. Experimental data were analyzed by the regression equation with Design Expert (Version 6.0.2) software. It indicated that the optimum extraction parameters are temperature 89.35 ℃, time 1.47 h and the ratio of solid to solution 1:29.19. Under these conditions, the predicted value of GLP extraction yield is 7.76 mg/g, in consistent with the measured value. It is the maximal extraction yield of GLP with optimized process parameters.

Key words: Ganoderma lucidum polysaccharides (GLP), response surface methodology (RSM), extraction yield