食品科学 ›› 2008, Vol. 29 ›› Issue (5): 311-314.
• 生物工程 • 上一篇 下一篇
李长美, 郭成金
出版日期:
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LI Chang-Mei, GUO Cheng-Jin
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摘要： 以玉米渣和葡萄糖为复合碳源,以麸皮和蛋白胨及酵母粉为复合氮源,采用L9(34)正交设计法优化桑黄菌丝体液体培养基,并为此初步确定了液体培养条件。实验结果表明:最佳培养基为玉米渣3%、麸皮3%、葡萄糖3%,KH2PO40.3%、MgSO4·7H2O0.15%、VB120μg/100ml、VB230μg/100ml。在32.5℃、pH自然、摇床转速130r/min、摇床培养192h的条件下,其菌丝体生物量平均得率为4.4g/100ml。
关键词: 桑黄, 液体培养基, 正交设计, 优化, 菌丝体, 生物量
Abstract: Corn flour as mixed carbonic source and bran+peptone+yeast as mixed nitrogenous source,the liquid culture medium of Phellinus linteus mycelia was optimized by orthogonal test in this study.The results showed that the optimal culture medium comprised corn flour 3%,bran 3%,glucose 3%,KH2PO4 0.3%,MgSO4·7H2O 0.15%,VB1 20μg/100 ml,VB2 30μg/100 ml,and on the culture conditions,of culture temperature 32.5 ℃,natural pH,rotation speed 130 r/min and culture cycle 192 h,the average mycelial biomass was 4.4 g/100 ml.
Key words: Phellinus linteus, liquid culture medium, orthogonal test, optimiztion, mycelia, biomass
李长美, 郭成金. 采用正交设计法对桑黄菌丝体液体培养基的优化[J]. 食品科学, 2008, 29(5): 311-314 https://doi.org/
LI Chang-Mei, GUO Cheng-Jin. Optimization of Liquid Culture Medium for Phellinus linteus Mycelia by Orthogonal Test[J]. FOOD SCIENCE, 2008, 29(5): 311-314 https://doi.org/
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