食品科学 ›› 2007, Vol. 28 ›› Issue (7): 360-361.
• 分析检测 • 上一篇 下一篇
周维义, 邓小娟
出版日期:
发布日期:
ZHOU Wei-Yi, DENG Xiao-Juan
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摘要： 目的:建立三氯蔗糖高效液相色谱测定方法。方法:采用DIKMAC18色谱柱(200mm×4.6mmi.d.5μm),流动相为甲醇-水(体积比为65:35),流速1.0ml/min,示差折光检测器,柱温40℃。结果:三氯蔗糖质量浓度在1.5～2000mg/L范围内与峰面积呈良好的线性关系,平均回收率为99.65%,相对标准偏差(RSD)为0.15%。结论:方法简便快速,操作稳定,经济环保,适用于三氯蔗糖的分析。
关键词: 高效液相色谱, 三氯蔗糖, 示差检测器
Abstract: Objective: A high performance liquid chromatographic method was developed to determine sucralose. Method: The column of DIKMA C18 (250 mm × 4.6 mm i.d. 5 μm) and a mobile phase of methanol-water (65:35, V/V) at a flow rate of 1.0 ml/min and column temperature 40 ℃ with refractive index detector were adopeted. Results: The method is proved to be linear in the range of sucralose from 1.5～2000 mg/L. The recovery is 99.65%. The relative standard deviation (RSD) is 0.15%. Conclusion: The method is simple, fast and stable. It is suitable for quantitative analysis for sucralose production process.
Key words: high performance liquid chromatography, sucralose, refractive index detector
周维义, 邓小娟. 高效液相色谱法分析三氯蔗糖的含量[J]. 食品科学, 2007, 28(7): 360-361 https://doi.org/
ZHOU Wei-Yi, DENG Xiao-Juan. Determination of Sucralose by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2007, 28(7): 360-361 https://doi.org/
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