食品科学 ›› 2007, Vol. 28 ›› Issue (8): 294-296.

• 生物工程 • 上一篇    下一篇

蝉拟青霉产透明质酸最佳液体培养基的筛选

 谭艾娟, 武立琨, 余晓蓓   

  1. 贵州大学生命科学学院; 贵州大学生命科学学院 贵州贵阳550025; 贵州贵阳550025;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Screen of Optimum Broth of Paecilomyces cicadae for Producing Hyaluronic Acid

 TAN  Ai-Juan, WU  Li-Kun, YU  Xiao-Bei   

  1. College of Life Science, Guizhou University, Guiyang 550025, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 本研究利用硫酸咔唑法检测透明质酸含量,考察了碳源、氮源和无机盐对蝉拟青霉发酵液中透明质酸含量的影响;实验结果表明,蝉拟青霉产透明质酸的最佳碳源、氮源和无机盐分别为:6%的乳糖、0.5%的牛肉膏与酵母膏的混合物(1:1)以及0.1%的MgSO4·7H2O。

关键词: 蝉拟青霉, 透明质酸, 培养基, 硫酸咔唑

Abstract: The effects of carbon source, nitrogen source and mineral salt on hyaluronic acid (HA) concentration in Paecilomyces cicadae broth were determined by the method of sulfuric acid carbazole. The results showed that the optimum liquid medium formula of Paecilomyces cicadae for producing HA is lactose (6%), mixture of beef extract (0.5%) and yeast extract(at the ratio of 1:1) and MgSO4·7H2O (0.1%).

Key words: Paecilomyces cicadae, hyaluric acid, culture medium, sulfuric acid carbazole