食品科学 ›› 2024, Vol. 45 ›› Issue (9): 36-43.doi: 10.7506/spkx1002-6630-20230627-211

• 食品化学 • 上一篇    下一篇

壳聚糖-透明质酸钠-益生菌水凝胶的制备、表征及胃肠道缓释作用

厉佳怡,王红磊,李娅婕,郭婷婷,倪乙丹,周泉城,盛桂华   

  1. (山东理工大学农业工程与食品科学学院,山东 淄博 255200)
  • 出版日期:2024-05-15 发布日期:2024-05-01
  • 基金资助:
    山东省重点研发计划项目(2019GNC106084)

Preparation and Characterization of Chitosan-Sodium Hyaluronate-Probiotic Hydrogels and Its Sustained-Release Performance in Simulated Gastrointestinal Tract Conditions

LI Jiayi, WANG Honglei, LI Yajie, GUO Tingting, NI Yidan, ZHOU Quancheng, SHENG Guihua   

  1. (College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255200, China)
  • Online:2024-05-15 Published:2024-05-01

摘要: 以壳聚糖(chitosan,CS)和透明质酸钠(sodium hyaluronate,SH)为原料,通过静电相互作用构建物理交联水凝胶,并对其质构特性及微观结构进行分析表征;以水凝胶为载体荷载益生菌(鼠李糖乳杆菌(Lactobacillus rhamnosus)以及乳酸片球菌(Pediococcus acidilactici)),探究益生菌对水凝胶质构特性及微观结构的影响,分析其载菌性能,探究益生菌水凝胶在模拟胃肠液中的释放机制。结果表明,CS-SH水凝胶通过静电相互作用交联,具有较好的质构特性以及载菌性能,0.2 g(干质量)水凝胶对L. rhamnosus及P. acidilactici荷载量分别可达1.15×109 CFU和1.25×109 CFU;益生菌水凝胶组在模拟肠液中持续释放,其释放机制为表面侵蚀释放作用,模拟肠液中L. rhamnosus及P. acidilactici活菌数最大值分别为6.30(lg(CFU/mL))和6.12(lg(CFU/mL))。综上,CS-SH水凝胶是一种具有潜力的递送载体,可以荷载益生菌并在模拟肠液中持续释放。本研究可为物理交联水凝胶在荷载益生菌领域的应用及作用机理提供一定理论指导及研究基础。

关键词: 物理交联水凝胶;壳聚糖;透明质酸钠;载菌性能;胃肠消化模拟

Abstract: A physically cross-linked hydrogel was constructed by electrostatic interaction between chitosan (CS) and sodium hyaluronate (SH), and its texture properties, microstructure and functional properties were characterized. Meanwhile, the influence of loading with one of the probiotics Lactobacillus rhamnosus and Pediococcus acidilactici on texture characteristics and microstructure of the hydrogel was evaluated, the loading performance was analyzed, and the release mechanism of probiotic-loaded hydrogels in simulated gastroenteric fluid was explored. The results showed that CS-SH hydrogel, which was formed through electrostatic crosslinking, had good texture properties and bacteria-loading properties, and the loading capacity of 0.2 g (dry mass) of hydrogels for L. rhamnosus and P. acidilactici was 1.15 × 109 and 1.25 × 109 CFU, respectively. The probiotic-loaded hydrogel was continuously released in simulated intestinal fluid, and its release mechanism was through surface erosion. The maximum viable counts of L. rhamnosus and P. acidilactici in simulated intestinal fluid were 6.30 and 6.12 (lg(CFU/mL)), respectively. To sum up, CS-SH hydrogel is a potential probiotic delivery carrier that can be continuously released in simulated intestinal fluid. This study provides theoretical guidance and a research basis for the application and mechanism of action of physically crosslinked hydrogels in the field of probiotic loading.

Key words: physically cross-linked hydrogels; chitosan; sodium hyaluronate; bacteria-loading performance; simulated gastrointestinal digestion

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