食品科学 ›› 2005, Vol. 26 ›› Issue (1): 67-70.

• 基础研究 • 上一篇    下一篇

粗糙脉孢菌(Neurosporacrassa)产纤维素酶发酵条件研究

 冯炘, 王丹, 辛丽霞, 宋文华   

  1. 天津理工学院环境科学与安全工程学院; 山东农业大学理学院; 南开大学环境科学与工程学院; 天津理工学院环境科学与安全工程学院
  • 出版日期:2005-01-15 发布日期:2011-09-19

Study on Fermentation Conditions of Cellulase Produced by Neurosoora crassa

 FENG  Xin, WANG  Dan, XIN  Li-Xia, SONG  Wen-Hua   

  1. 1.Department of Environment Science and Safe Engineering,Tianjin Institute of Technology;2.Department of Technology,Shandong Agricultural University;3.Department of Environment Science and Engineering,Nankai University
  • Online:2005-01-15 Published:2011-09-19

摘要: 本文对粗糙脉胞菌Neurosporacrassa1602摇瓶发酵产纤维素酶的条件进行了研究。结果表明:培养液初始pH5.5,培养温度28℃,摇瓶转速150r/min,培养96h,诱导产酶碳源为3%玉米芯粉+0.5%麸皮酸水解液(0.6mol/L),有机氮源为黄豆粉或无机氮源为NH4HSO4时为最佳产酶条件。其纤维素酶活力CMCase为10.34IU/ml,FPase为0.71IU/ml。

关键词: 粗糙脉胞菌, 纤维素酶, 发酵, 条件

Abstract: In this paper,fermentation conditions of cellulase produced by Neurospora crassa in the shaking bottle were studied. The results showed that the optimum conditions were as follows: cultivating solution initial pH was 5.5, cultivating temperature 28℃, rotation speed 150r/min and cultivating time 96 h. The most suitable organic nitrogen source was soybean powder and the most suitable inorganic nitrogen source was NH4HSO4. It helped produc cellulase under the conditions of 3% corncob powder and 0.5% bran acid hydrolysis solution(0.6 mol/L).In the optimum conditions, CMCase activity was 10.34IU/ml and FPase was 0.71IU/ml.

Key words: Neurospora crassa, cellulase, fermentation, condition