食品科学 ›› 2005, Vol. 26 ›› Issue (3): 39-42.

• 基础研究 • 上一篇    下一篇

不同来源免疫球蛋白G的体外抑菌效果

 罗明姬, 曹劲松, 彭志英   

  1. 华南理工大学食品与生物工程学院
  • 出版日期:2005-03-15 发布日期:2011-09-19

Study on Antibacterial Effect in Vitro of Immunoglobulin G from Different Sources

 LUO  Ming-Ji, CAO  Jin-Song, PENG  Zhi-Ying   

  1. College of Food Scienc and Biotechnolgy, South China University of Technology
  • Online:2005-03-15 Published:2011-09-19

摘要: 本文研究4种不同来源的IgG制品对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑菌作用。采用液体培养基连续稀释法对不同来源IgG做定性抑菌实验;分别培养12、24h后将其做定量平板涂布实验。30℃恒温培养下,牛初乳IgG、牛血IgG及猪血IgG对以上三种菌均具有一定抑制作用,12h的抑菌效果更显著。在本实验条件下未观察到鸡蛋IgY对上述3种细菌的抑菌效果。37℃下,仅观察到牛初乳IgG在80mg/ml浓度下对于St. aureus表现出一定抑菌作用。

关键词: 抑菌作用, 牛初乳IgG, 牛血IgG, 猪血IgG, 鸡蛋IgY

Abstract: The inhibition of immunoglobulin G from different sources on Escherichia coli, Staphylococcus aureus and Bacillus subtilis was studied respectively in this article. The qualitative inhibition of IgG on the three germs determined by tubes serial dilution test and quantitative newcombe experiment was made respectively after 12h and 24h of the fluid culture. Under 30℃, it showed that bovine colostrum IgG, bovine serum IgG and swine serum IgG inhibited respectively the three germs to some extent, and the antibacterial effect of those IgG in 12h culture was more remarkable. But chicken IgY did not inhibit any germ under the experiment condition. Under 37℃, it was observed that bovine colostrum IgG had only low inhibiting rate on St. aureus at 80mg/ml concentration.

Key words: antibacterial effect in vitro, bovine colostrum IgG, bovine serum IgG, swine serumIgG, chicken IgY