鼠李糖乳杆菌培养基成分的快速筛选(英文)

食品科学 ›› 2007, Vol. 28 ›› Issue (11): 382-385.

鼠李糖乳杆菌培养基成分的快速筛选(英文)

于雷, 雷霆, 裴晓林

吉林农业大学食品科学与工程学院；吉林大学分子酶学工程教育部重点实验室；吉林大学分子酶学工程教育部重点实验室吉林长春130118；吉林长春130021；

出版日期:2007-11-15 发布日期:2011-11-22

Rapid Screen of Medium Components for L(+)Lactic Acid Production by Lactobacillus rhamnosus

YU Lei, LEI Ting, PEI Xiao-Lin

1.College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;2.Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130023, China

Online:2007-11-15 Published:2011-11-22

摘要/Abstract

摘要： 本实验利用单因素试验和Plackett-Burman试验快速筛选鼠李糖乳杆菌(L.rhamnosusMEE539)的培养基成分。首先,采用单因素试验分别从碳源和氮源中筛选出影响显著的成分,然后将这些成分连同表面活性剂和矿物质进行Plackett-Burman试验。结果表明,在27种被测成分中葡萄糖、糖蜜、酵母粉、玉米浆、土温80和硫酸锰对鼠李糖乳杆菌发酵的影响最显著,置信度均大于95%。单因素试验和Plackett-Burman试验相结合可以降低试验劳动强度,减少培养基配制的差错,有助于不同种类培养基成分的快速筛选。

关键词: 鼠李糖乳杆菌:培养基成分, Plackett-Burman试验设计, 单因素试验

Abstract: The Plackett-Burman design was applied in cooperation with the single factor test for rapid screening the medium components for L (+) lactic acid production by L.rhamnosus MEE539.The most significant influencing factors carbon and nitrogen sources were screened by the single factor test and then together with surfactant and minerals were investigated in the subsequent Plackett-Burman design. The analysis results demonstrated that glucose, molasses, yeast extract, corn steep liquor, Tween80 and MnSO4 have the greatest impacts on the lactic acid production at a statistical confidence greater than 95% from 27 variables. In the present work, it was evident that the cooperation between Plackett-Burman design and single factor test allowed the quick identification of the most significant medium components for lactic acid production. Furthermore, this method proved to be useful in saving the researchers’ labor and reducing the errors occurred in the process of making medium.

Key words: Lactobacillus rhamnosus, medium components, Plackett-Burman design, single factor test

于雷, 雷霆, 裴晓林. 鼠李糖乳杆菌培养基成分的快速筛选(英文)[J]. 食品科学, 2007, 28(11): 382-385.

YU Lei, LEI Ting, PEI Xiao-Lin. Rapid Screen of Medium Components for L(+)Lactic Acid Production by Lactobacillus rhamnosus[J]. FOOD SCIENCE, 2007, 28(11): 382-385.

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