关键词: 多残留分析, 喹诺酮类, 鸡肉, 高效液相色谱法

Abstract: A reversed-phase high performance liquid chromatographic method has been developed for the simultaneous determination of four quinolone antimicrobial residues in chicken. Quinolone antimicrobial were extracted with acetonitrile, followed by hexane partitioning. After the solvent was vaporized with a rotary evaporator, Quinolone antimicrobials were analyzed by HPLC-FLD and quantified by external standard method. The liner range was 0.05～1.2μg/ml, and the correlation coefficients were 0.9997～0.9999. The average recoveries of four quinolone antimicrobials in spiked chicken ranged from 78.3% to 101.8%, and the relative standard deviations were between 3.89% and 12.43%. The limit of detection was 0.0037mg/kg for ofloxacin, 0. 0037mg/kg for norfloxacin, 0.0035mg/kg for ciprofloxacin and 0.0022mg/kg for enrofloxacin. The results have indicated that this multiresidues developed were easier, faster. It has also demonstrated that this multiresidue analysis method could meet the requirements for simultaneous determination of many quinolone antimicrobials in chicken.

Key words: multiresidue analysis, quinolone, chicken, HPLC