食品科学 ›› 2009, Vol. 30 ›› Issue (6 ): 128-131.doi: 10.7506/spkx1002-6630-200906026

• 工艺技术 • 上一篇    下一篇

SD3 离子交换树脂分离提取ATP 的研究

乔德阳,甘 聃   

  1. 徐州工业职业技术学院化学工程系
  • 收稿日期:2008-12-30 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 乔德阳 E-mail:qiaodeyang@sohu.com

Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin

QIAO De-yang,GAN Dan   

  1. (Department of Chemical Engineering, Xuzhou College of Industrial Technology, Xuzhou 221140, China)
  • Received:2008-12-30 Online:2009-03-15 Published:2010-12-29
  • Contact: QIAO De-yang E-mail:qiaodeyang@sohu.com

摘要:

通过对SD3 离子交换树脂对ATP 动态吸附、洗杂和洗脱条件的考察,确定最适吸附条件为:样品溶液H 2.0,浓度8.25g/L,离子交换柱的高径比为10(装柱湿树脂80g),流速1.0ml/min;最优洗杂液为pH1.5 HC1+ 0.05mol/L NaCl 的溶液;ATP 的最优洗脱液为pH12.0 NaOH+0.1mol/L NaCl 溶液,优洗脱速度为0.5ml/min。

关键词: 三磷酸腺苷(ATP), 离子交换树脂, 分离

Abstract:

The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min.

Key words: ATP, ion exchange resin, separation

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