莱克多巴胺的抗原合成鉴定及其抗体的制备纯化

食品科学 ›› 2005, Vol. 26 ›› Issue (12): 29-33.

• 基础研究 • 下一篇

莱克多巴胺的抗原合成鉴定及其抗体的制备纯化

许文涛, 黄昆仑, 邓爱科, 罗云波

中国农业大学食品科学与营养工程学院

出版日期:2005-12-15 发布日期:2011-10-01

The Synthesis and Identification of the Antigen of RCT and the Production and Purification of Its Polyclonal Antibody

XU Wen-Tao, HUANG Kun-Lun, DENG Ai-Ke, LUO Yun-Bo

College of Food Science and Nutritional Engineering, China Agricultural University

Online:2005-12-15 Published:2011-10-01

摘要/Abstract

摘要： 莱克多巴胺属于β-兴奋剂类药物中的苯乙醇类衍生物,具有显著改善动物饲料利用效率,显著提高胴体瘦肉率的生产性能,因为其具有严重的副作用所以目前该类药物在我国和其他国家是被禁用的。本试验通过连接剂butane-1,4-dioldiglyciydylether把莱克多巴胺和载体蛋白BSA和OVA偶联成免疫抗原和包被抗原,并采用紫外全波长扫描和SDS-聚丙烯酰胺凝胶的方法对合成的抗原进行了鉴定。通过免疫兔子获得了RCT的多克隆抗体,然后又通过饱和硫铵沉淀和proteinA-Sepharose4B的方法对抗体进行了纯化。纯化的抗体表现出对RCT较高的特异性和灵敏性。

关键词: 莱克多巴胺, 免疫抗原, 包被抗原, 多克隆抗体, 酶联免疫检测技术

Abstract: Ractopamine (RCT) is a phenethanolamine member of the family of β-adrenergic agonists(β-agonists). This class of compounds has become notable for their properties of enhancing the growth rates of farm animal species but is not licensed for use in China and other countries because of their serious side-effects. The RCT is coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent, butane-1,4-diol diglyciydyl ether. The immunogen and coating antigen have been identified by the method of ultraviolet full wavelength scanner and SDS-PAGE. Polyclonal antibodies against RCT have been raised in three rabbits and purified by using a saturated ammonium sulfate method and by the way of protein A-Sepharose 4B. The sensitivity and cross-reactivity of polyclonal antibody have been demonstrated in ELISA test. The purified polyclonal anti- RCT antiserum shows a very high degree of sensitivity and specificity.

Key words: Ractopamine, immunogen, coating antigen, polyclonal antibody, ELISA

许文涛, 黄昆仑, 邓爱科, 罗云波. 莱克多巴胺的抗原合成鉴定及其抗体的制备纯化[J]. 食品科学, 2005, 26(12): 29-33.

XU Wen-Tao, HUANG Kun-Lun, DENG Ai-Ke, LUO Yun-Bo. The Synthesis and Identification of the Antigen of RCT and the Production and Purification of Its Polyclonal Antibody[J]. FOOD SCIENCE, 2005, 26(12): 29-33.

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莱克多巴胺的抗原合成鉴定及其抗体的制备纯化

The Synthesis and Identification of the Antigen of RCT and the Production and Purification of Its Polyclonal Antibody

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