β-1,3葡聚糖酶高产菌株的筛选及其产酶条件研究

食品科学 ›› 2004, Vol. 25 ›› Issue (5): 31-35.

β-1,3葡聚糖酶高产菌株的筛选及其产酶条件研究

陈福生, 王勇, 陈九武

华中农业大学食品科技学院

出版日期:2004-05-15 发布日期:2011-10-24

Study on High β-1,3-glucanase Strain Screening from Soil and Enzyme Producing Conditions

CHEN Fu-Sheng, WANG Yong, CHEN Jiu-Wu

College of Food Science and Technology, Huazhong Agricultural University

Online:2004-05-15 Published:2011-10-24

摘要/Abstract

摘要： 从土壤中分离筛选出一株β-1,3葡聚糖酶活较高的菌株,编号为M014,初步鉴定为木霉。研究了碳源、氮源、培养温度与时间等因素对M014产酶的影响。实验结果表明,M014在含3%茯苓粉、0.5%酵母膏及一些无机盐的液体培养基中(pH6.0),于30℃,150r/min振摇培养6d,β-1,3葡聚糖酶活最高,达到4.95U/ml。另外,还就β-1,3葡聚糖酶的酶解条件进行了研究,结果显示,β-1,3葡聚糖酶的最适反应pH为4.5,最适反应温度为60℃。粗酶液在40℃和50℃保温12h,酶活基本稳定,60℃保温时,酶活迅速下降。

关键词: &beta, -1, 3葡聚糖酶, 菌株筛选, 产酶条件

Abstract: A strain M014, identified as Trichoderma sp., with high β-1,3-glucanase was isolated from the soil. The effects ofthe different nutrients (carbon source and nitrogen source) and cultural conditions (temperature and time) on β-1,3-glucanaseproduction by M014 were studied. The maximal activity of β-1,3-glucanase was reached at 30℃ at 150r/min with 6d incubationin a liquid medium(pH6.0), containing 3% Fuling powder, 0.5% yeast cream and mineral salts. Enzymatic properties of the crudeenzyme filtration from M014 were investigated. The optimal pH and temperature for the enzyme reaction were pH4.5 and60℃, respectively. At the temperature of 40℃ and 50℃ to be preserved for 12h, the activity of β-1,3 glucanase was still stableHowever, over the temperature of 60℃, it descended rapidly.

Key words: glucanase, screening strain, condition of enzyme production

陈福生, 王勇, 陈九武. β-1,3葡聚糖酶高产菌株的筛选及其产酶条件研究[J]. 食品科学, 2004, 25(5): 31-35.

CHEN Fu-Sheng, WANG Yong, CHEN Jiu-Wu. Study on High β-1,3-glucanase Strain Screening from Soil and Enzyme Producing Conditions[J]. FOOD SCIENCE, 2004, 25(5): 31-35.

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