食品科学 ›› 2004, Vol. 25 ›› Issue (6): 156-159.

• 分析检测 • 上一篇    下一篇

姜黄素的高效薄层色谱分析

 徐仲溪, 王坤波   

  1. 湖南农业大学食品科技学院,湖南省天然产物工程技术研究中心
  • 出版日期:2004-06-15 发布日期:2011-10-24

Analysis of Curcumin by High Performance Thin-Layer Chromatoraphy(HPTLC)

 XU  Zhong-Xi, WANG  Kun-Bo   

  1. 1.College of Food Science and Technology,Hunan Agricultural University;2.Hunan Provincial Engineering Research Center for Natural Products
  • Online:2004-06-15 Published:2011-10-24

摘要: 以硅胶GF254板为固定相、氯仿-甲醇-甲酸(体积比为96:4:0.7)为流动相,建立了姜黄素薄层色谱分析方法。姜黄素样品溶液上行展开5.0cm,其Rf值为0.70。姜黄素的检测量在27~216ng范围内,其斑点的面积与姜黄素的检测量具有良好的线性关系,相关系数为0.9983。姜黄素的平均回收率为102.08%,方法的精密度良好(RSD≤1.5%)。该方法简便、快速、准确,可用于姜黄样品的定性定量分析。

关键词: 薄层色谱扫描法, 姜黄, 姜黄素, 薄层扫描

Abstract: The thin-layer chromatographic method of curcumin was developed by the use of chloroform-methanol-formic acid(96:4:0.7 in volume ratio) as a mobile phase and on GF254 silica gel plate. The Rf value of cuecumin was 0.70. The calibration curvewas linear (r=0.9983) in the range of 27~216ng of curcumin and the average recovery of 102.08%.The results showed it to bea simple, rapid sensitive and reproducible method for the determination of curcumin in curcuma samples and curcumin extract.

Key words: thin-layer chromatography, curcuma Longa, curcumin, thin-layer scan