食品科学 ›› 2004, Vol. 25 ›› Issue (7): 100-104.

• 工艺技术 • 上一篇    下一篇

牡蛎双酶水解工艺的研究

 付猛, 云霞, 朱蓓薇   

  1. 大连轻工业学院生物与食品工程学院
  • 出版日期:2004-07-15 发布日期:2011-10-24

Study on Bienzymatic Hydrolysis of Oyster

 FU  Meng, YUN  Xia, ZHU  Bei-Wei   

  1. College of Biology and Food Engineering ,Dalian Institute of Light Industry
  • Online:2004-07-15 Published:2011-10-24

摘要: 本文研究了双酶水解牡蛎蛋白的工艺条件。分析了酶用量、pH值、温度、双酶比例、反应时间等因素对牡蛎蛋白水解的影响。通过正交试验,确定其最佳水解条件为:温度45℃,pH值7.5,酶用量5000U/g,两种酶活力比(F:D)3:1,时间2h,底物浓度3%。在此条件下,水解度达28.68%,酸溶性肽得率达79.89%。牡蛎蛋白经双酶水解后的水解液可用于生产功能性食品。

关键词: 牡蛎, 蛋白酶, 双酶水解, 多肽

Abstract: In this paper, bienzymatic hydrolysis of oyster protein was studied. The effects of the main parameters includingenzyme dosage, pH, hydrolysis temperature, bienzyme ratio and hydrolysis time on hydrolysis reaction were investigated. Onthe basis of orthogonal tests, the optimum hydrolytic conditions were as follows:temperature 45℃, pH7.5, enzyme dosage5000U/g, the ratio of the two enzyme (F:D)3:1, hydrolysis time 2h and substrate concentration 3%. Under the conditions, thedegree of hydrolysis (DH) and the yield of acid-solubility peptide(YASP) were 28.68%and 79.89% respectively. The hydroly-sate product of oyster protein could be used to produce functional food.

Key words: oyster, protease, bienzymatic hydrolysis, poly-peptide