食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 194-196.doi: 10.7506/spkx1002-6630-200919043

• 生物工程 • 上一篇    下一篇

肉源产蛋白酶葡萄球菌发酵剂的筛选研究

董 杰,蒋云升*   

  1. 扬州大学旅游烹饪学院
  • 收稿日期:2009-06-04 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 蒋云升
  • 基金资助:

    江苏省科技厅技术创新基金项目(BN2008044);南通市科技创新计划项目(AL2007002)

Screening of High-yield Protease-producing Staphylococcus from Traditional Fermented Meat Products

DONG Jie,JIANG Yun-sheng*   

  1. College of Tourism and Culinary Science, Yangzhou University, Yangzhou 225001, China
  • Received:2009-06-04 Online:2009-10-01 Published:2010-12-29
  • Contact: JIANG Yun-sheng

摘要:

从如皋火腿、香肠、金华火腿、宣威火腿等代表性样品分离得到的G+球菌中,有15株在酪蛋白双层鉴别培养基中产生蛋白水解圈。通过接种MSA培养基与摇床发酵,得到蛋白酶活力最高的为C8菌株,经过系统生理生化鉴定表明该菌株为表皮葡萄球菌(S. epidermidis),并对其产酶温度等发酵条件进行初步研究。

关键词: 发酵肉制品, 表皮葡萄球菌, 蛋白酶活性, 筛选

Abstract:

Gram positive Staphylococcus was isolated from representative samples of Rugao ham and sausage, Jinhua ham and Xuanwei ham. Totally 15 strains were screened to have capability of hydrolyzing casein. Based on physiological and biochemical identification, the selected strain C8 with highest protease activity was S. epidermidis.

Key words: fermented meat products, Staphylococcus epidermidis, protease activity, screening

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